nowadays gamete and embryo freezing is an appropriate approach for preserving of genetic traits in laboratory animals, rare and endangered species. frozen cells are suitable replace for actively breeding animals colony. the aim of this study was to perserve laboratory mouse embryo, using vitrification method and comparing effect of two cryoprotectants, glycerol-sucrose(gs) and ethylene glycol-f...

nowadays gamete and embryo freezing is an appropriate approach for preserving of genetic traits in laboratory animals, rare and endangered species. frozen cells are suitable replace for actively breeding animals colony. the aim of this study was to perserve laboratory mouse embryo, using vitrification method and comparing effect of two cryoprotectants, glycerol-sucrose(gs) and ethylene glycol-f...

many investigators are interested in finding the new cultural systems that can support the in vitro development of pre-implantation embryos better. previous studies suggested that growth factors such as epidermal growth factor (egf) are important in pre-implantation embryo development and implantation process. on the other hand, it is very important to support post thaw development of frozen em...

Many investigators are interested in finding the new cultural systems that can support the in vitro development of pre-implantation embryos better. Previous studies suggested that growth factors such as epidermal growth factor (EGF) are important in pre-implantation embryo development and implantation process. On the other hand, it is very important to support post thaw development of frozen em...

introduction: the objective of this study was to assess developmental potential of mouse morulae, early and laic blastocysts after vitrification in r2 and r2+vero mediums. material and methods: morulae, early and late blastocysts were obtained from superovulated nmri female mice. embryos in experimental group were suspended in a solution of ethylene glycol, ficoll and sucrose (efs40) for 2 minu...

AIMS To characterize the relationship between the sucrose- and ethanol-reinforced behaviour in Wistar rats. METHODS Subjects (n = 31) were trained to lever press for 8% (v/v) ethanol in an operant procedure where increasing concentrations of ethanol were introduced in the presence of 8% (w/v) sucrose. The sucrose concentration was subsequently decreased from 8 to 0%. Subjects were allowed to ...