The antituberculosis activity of subtilin, an antibiotic agent introduced by Jansen and Hirschmann (1944), has been the subject of conflicting reports in the literature. Salle and Jann (1945) reported that subtilin in a dilution of 1:50,000 prevented surface growth of Mycobacterium tuberculosis on Long's synthetic medium, and that transfers into fresh medium made from tubes containing 1:10,000 ...

Lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. Autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. In this report, we used the previously reported subtilin autoinduction bioassay in combination with mass spectrometric ana...

The production of the type I antimicrobial peptide (AMP) subtilin by Bacillus subtilis is regulated in a cell-density-dependent manner [Kleerebezem M, de Vos WM, Kuipers OP. The lantibiotics nisin and subtilin act as extracellular regulators of their own biosynthesis. In: Dunny GM, Winans SC, editors. Cell-cell signaling in bacteria. Washington, D.C., USA: ASM Press; 1999. p. 159-74; Stein T, B...

The early literature on the effect of the polypeptide antibiotics subtilin and nisin on bacterial spores has been reviewed by Campbell and O'Brien (1955). Most of the early reports have indicated that subtilin acts as a sporostatic agent. Other reports have shown, however, that the spores of several bacterial species will germinate in the presence of subtilin and are subsequently killed by the ...

Several investigations have revealed that Clostridium botlelinum is sensitive to the polypeptide antibiotic subtilini (Andersen, 1952; Williams and Fleming, 1952; Andersen et al., 1953; Le Blanc et al., 1953; Lewis et al., 1954; Michener, 1955; O'Brien et al., 1956). For this and other reasons, subtilin has been extensively studied for its potential use ill the preservation of canned foods (Cam...

Subtilin is an antibiotic extracted from the cells of a particular strain of Bacillus subtiliws.3" It is a low molecular weight substance that dialyzes through collodion membranes, is sensitive to light and alkali, and has the structure of a peptide. Subtilin is soluble to at least 30 per cent in saltfree acidified solution. The solubility in acid solution is markedly decreased by the presence ...

This report demonstrates the usefulness of PCR for the genes spaS and sboA as a means of identifying Bacillus strains with a potential to produce subtilin and subtilosin A. One collection strain and five Bacillus spp. isolated from aquatic environments in the Amazon basin were screened by PCR using primers for sboA and spaS designed specifically for this study. The sequences of the PCR products...

The genetic network regulating the biosynthesis of subtilin in Bacillus subtilis is modeled as a stochastic hybrid system. The continuous state of the hybrid system is the concentrations of subtilin and various regulating proteins, whose productions are controlled by switches in the genetic network that are in turn modeled as Markov chains. Some preliminary results are given by both analysis an...

Sequence analysis upstream from the subtilin structural gene (spaS) in Bacillus subtilis ATCC 6633 revealed several open reading frames, SpaB, SpaC, and SpaD. SpaB, consisting of 599 amino acid residues, shows excellent homology with a variety of membrane translocator proteins, such as HlyB from Escherichia coli and some mammalian multidrug resistance proteins. When the spaB gene was interrupte...

This paper presents methods for the parameter identification of a model of subtilin production by Bacillus subtilis. Based on a stochastic hybrid model, identification is split in two subproblems: estimation of the genetic network regulating subtilin production from gene expression data, and estimation of population dynamics based on nutrient and population level data. Techniques for identifica...