orchids are one of the most popular plants in the world and among them phalaenopsis genus have the most sales in a global market. because of its hard propagation, micropropagation technique has been employed recently. in this study, we tried to find out useful protocol for commercial production through in vitro culture. at first, for seed capsules production, flowers were pollinated with hand i...

ginger (zingiber officinale rosc.) is a herbaceous, rhizomatous, perennial, which its rhizomes are used as an important spices and medicine all over the world. the aromatic principles include zingiberine, while the pungent principles are known as gingeroles. callus culture of the plants can produce the same compounds exist on their parent plants. the aim of this work was to evaluate the effect ...

Stevia rebaudiana Bertoni, member of Asteraceae family, has bio-active compounds stevioside and rebaudioside which taste about 300 times sweeter than sucrose. It regulates blood sugar, prevents hypertension and tooth decay as well as used in treatment of skin disorders having high medicinal values, and hence there is a need for generating the plant on large scale. We have developed an efficient...

An in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by KC medium. After 40 days, all the media turned brown and the growths of the protocorms were arrested. Activated charcoal, 1 g/l ...

Thidiazuron (1-phenyl-3(1,2,3Thiadiazol-5-yl) urea; TDZ) is one of the several substituted ureas that have been investigated recently for their cytokinin-like activity. TDZ is known to be more active than zeatin for stimulating the growth when added to a tissue culture medium at a low concentration. In this study, effect of TDZ on several In vitro growth parameters in Solanum tuberosum cvs. Des...

the effect of in planta tiba and proline on in vitro seedlings and cell culture of sugar beet was investigated. sterilized seeds were grown in vitro on ½ strength ms medium supplemented with 0 or 3 mg/l tiba. calli obtained on young leaves cultured on ms medium containing 1 mg/l bap, were used for the intiation of cell suspension cultures using ms basal composition supplemented with 0 or 50 m m...

strawberry (fragaria × ananassa duchesne) is produced on 3500 ha in iran. during surveys on strawberry root and crown rot disease (february-april 2007), a fungus resemble macrophomina was isolated from crowns and roots of collapsed and dying strawberry plants which were collected from golestan, mazandaran and kordestan provinces of iran. to study rate of growth and cultural characteristic of is...

an in vitro plant regeneration protocol was successfully established in aphyllorchis montana , a saprophytic achlorophyllous orchid by culturing immature seeds. among the six basal media evaluated for seed germination, bm-tm medium was found to be the best followed by kc medium. after 40 days, all the media turned brown and the growths of the protocorms were arrested. activated charcoal, 1 g/l ...