background: direct identification of leishmania species in giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. this study was designed to isolate leishmani spp. from giemsa-stained smears and to characterize them by pcr technique. methods: a total of 48 giemsa-stained slides from confirmed cases of leishmaniasis were examined under a ligh...

In this work we present an automatic algorithm for detection of mycobacterium tuberculosis from stained sputum smear images applying a novel method of tubeness filtering in conjunction with Otsu thresholding and connected component analysis. This eliminates human effort and measurement error from the detection of the bacteria, which normally requires a lab technician or pathologist to load a gl...

Assessment of platelet count is an important diagnostic parameter in haematology. Automated blood cell counters have largely replaced the manual method. However, all abnormal platelet counts are verified in Leishman's stained peripheral blood smear. Platelets also can be identified in the unstained blood smears. The objective of this study is to compare the unstained peripheral blood smears wit...

In the present study, we investigated the influence of cytological stains in analyzing DNA extracted from cytological slides by comparative genomic hybridization (CGH). Multiple imprint cytological slides were prepared for fresh-frozen breast cancer tissue samples and the slides were stained by three staining methods for each sample. Under microscopic observation, cancer cells were selectively ...

Malaria presents a diagnostic challenge in most tropical countries like India. Microscopy remains the gold standard for diagnosing malaria, but it is labour intensive and depends upon the skill of the examiner. Malaria rapid diagnostic tests (RDT’s) have been developed as an easy, convenient alternative to microscopy. The aim of this study was to correlate and compare the conventional diagnosti...

dermatophilosis is a bacterial skin infection and wet conditions like, raining and dipping predispose sheep to it. a great economic loss can be caused by this disease because of its effect on the quality of wool. in iran (near saveh), there was an outbreak of the disease in a herd and the clinical manifestation was different from that of typical dermatophilosis. diffuse wide alopecic area accom...

Dermatophilosis is a bacterial skin infection and wet conditions like, raining and dipping predispose sheep to it. A great economic loss can be caused by this disease because of its effect on the quality of wool. In Iran (near Saveh), there was an outbreak of the disease in a herd and the clinical manifestation was different from that of typical dermatophilosis. Diffuse wide alopecic area accom...

Direct smear microscopy of sputum forms the mainstay of TB diagnosis in resource-limited settings. Stained sputum smear slides can serve as a ready-made resource to transport sputum for molecular drug susceptibility testing. However, bio-safety is a major concern during transport of sputum/stained slides and for laboratory workers engaged in processing Mycobacterium tuberculosis infected sputum...