a purification procedure of pertussis toxin (pt) from submerged culture of bordetella pertussis (b.pertussis) strain 134 using adsorption and affinity chromatography was discussed. the yield of the resulting pt was approximately 37.5mg/l of concentrated culture supernatant. the polypeptide pattern of the purified pt was investigated by sds-page and showed five bands between 11 to 26 kda using l...

Introduction: Hematopoiesis is an on going process mammalian marrow system. A few cells from the nucleated cells of bone marrow are hematopoietic cells which include primary stem cells, precursor cells and progenitor cells. Primary stem cells and progenitor cells are able to produce colonies in culture medium (CFU-C) and irradiated mouse spleen (CFU-S). A hematopoietic cell is alive and act...

Most common tumors of the spleen are hematologic and lymphoid malignancies. Non-lymphoid and non-hematologic tumors of the spleen are very rare, the most common of which are vascular tumors. This group of tumors in the spleen is composed of heterogeneous tumors such as hemangioma, angioendothelioma (AE), littoral cell angioma, and angiosarcoma. There are several histologic forms of AE such as e...

A man with acute myelogenous leukemia, treated with chemotherapy, had a decrease in circulating formed blood elements. Bone marrow biopsy revealed necrosis. Imaging with In-Ill chloride demonstrated activity in the liver, but not in the spleen or bone marrow. Eighteen days later, the circulating blood count had risen; a repeat bone marrow biopsy showed the return of blood cell precursors....

Follicular dendritic cell sarcomas (FDCS) are spindle lesions that large, slow-growing masses in either nodal or extranodal regions both and originate from B-cell follicles of the lymph nodes. Most tumors cervical nodes, but retroperitoneal mediastinal origins have also been reported. Extranodal areas include soft tissues, skin, tonsils, gastrointestinal tract, liver, spleen. The spleen is an u...

most common tumors of the spleen are hematologic and lymphoid malignancies. non-lymphoid and non-hematologic tumors of the spleen are very rare, the most common of which are vascular tumors. this group of tumors in the spleen is composed of heterogeneous tumors such as hemangioma, angioendothelioma (ae), littoral cell angioma, and angiosarcoma. there are several histologic forms of ae such as e...

ultrasonography is a useful procedure in evaluation of spleen size in different clinical conditions. in this study, we used it to evaluate spleen size in patients with various heptologic, hematologic and autoimmune diseases. to express spleen size, a spleen index (si), the product of the transverse diameter and its perpendiculr diameter measured on the maximum-sectional image of the spleen, was...