Introduction: Since Curcuma longa extract and curcumin have been shown to be potent antioxidant agents, they were used in cultured adult mouse spinal cord slices to investigate whether they can inhibit apoptosis in motor neurons. Methods: Slices from the thoracic region of adult mice spinal cord were divided into four groups: 1. Freshlyprepared slices (time 0) 2. Control 3. Slices treated by cu...

organotypic spinal cord slices from neonatal mammals could be a powerful model for evaluation of cell survival but also cell death mechanisms. the aim of this study was to establish an in vitro model for investigating cell survival and mechanism involved in cell death in neonatal spinal cord slices. the spinal cord was sliced and incubated into culture medium. the mtt assay was carried out to a...

objective: the apoptosis of motor neurons is a critical phenomenon in spinal cord injuries. adult spinal cord slices were used to investigate whether voltage sensitive calcium channels and na+/ca2+ exchangers play a role in the apoptosis of motor neurons. materials and methods: in this experimental research, the thoracic region of the adult mouse spinal cord was sliced using a tissue chopper an...

in this study organotypic adult spinal cord slices were used to investigate whether caspases could participate in the apoptosis of motor neurons. the thoracic region of spinal cord was sliced using a tissue chopper and cultured in a medium for 6h. morphological and biochemical features of apoptosis were assessed by fluorescent staining and terminal deoxynucleotidyl nick end labeling (tunel) met...

Networks generating locomotor-like rhythmic motor activity are formed during the last week of the fetal period in the rat spinal cord. We investigated the coordinated rhythmic motor activity induced in transverse slice preparations of the lumbar spinal cord taken from fetal rats as early as embryonic day (E) 16.5. In slices as thin as 100 microm, bath-application of 5-hydroxytryptamine (5-HT) i...

Prostatic acid phosphatase (PAP) and ecto-5'-nucleotidase (NT5E) hydrolyze extracellular AMP to adenosine in dorsal root ganglia (DRG) neurons and in the dorsal spinal cord. Previously, we found that adenosine production was reduced, but not eliminated, in Pap⁻/⁻/Nt5e⁻/⁻ double knock-out (dKO) mice, suggesting that a third AMP ectonucleotidase was present in these tissues. Here, we found that t...

Introduction: Today, clinicians and neuroscientists need to have a comprehensive survey of neurological pathologies and injuries. For the First-time, SEEP contrast and Spin-Echo pulse sequences was used for functional imaging of the Lumbar spinal cord. This method used by several research groups for Spinal cord mapping, but other researchers tried to improve BOLD fMRI to Spina...

Husch A, Cramer N, Harris-Warrick RM. Long-duration perforated patch recordings from spinal interneurons of adult mice. J Neurophysiol 106: 2783–2789, 2011. First published September 7, 2011; doi:10.1152/jn.00673.2011.—It has been very difficult to record from interneurons in acute slices of the lumbar spinal cord from mice 3 wk of age. The low success rate and short recording times limit in vi...

It has been very difficult to record from interneurons in acute slices of the lumbar spinal cord from mice >3 wk of age. The low success rate and short recording times limit in vitro experimentation on mouse spinal networks to neonatal and early postnatal periods when locomotor networks are still developmentally immature. To overcome this limitation and enable investigation of mature locomotor ...

Introduction: Since Curcuma longa extract and curcumin have been shown to be potent antioxidant agents, they were used in cultured adult mouse spinal cord slices to investigate whether they can inhibit apoptosis in motor neurons. Methods: Slices from the thoracic region of adult mice spinal cord were divided into four groups: 1. Freshlyprepared slices (time 0); 2. Control; 3. Slices treated by ...