objective: antioxidants are essential for sperm motility. calligonum extract possesses the important antioxidants catechin and quercetin. this study investigates the effects of calligonum extract on sperm parameters and the rate of apoptosis in testes of aging male mice. methods: we initially performed a dose response test with using three doses of calligonum (10 mg/kg, 30 mg/kg and 50 mg/kg). ...

background: in previous investigations, it has been clarified that electromagnetic fields (elf) can cause some changes in cellular behavior. the aim of this prospective study was to investigate the effect of magnetic field (mf) on human sperm parameters of motility, morphology, and viability. materials and methods: semen samples were collected from 12 fertile men, and were allowed to liquefy fo...

background: the lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. the purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (mda) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. in addition, correlation of lipid peroxidation with mot...

AIM To investigate whether early apoptotic changes in spermatozoa can be significant markers for sperm quality. METHODS Two early apoptotic changes in the semen of 56 men were assessed using Annexin V (AN)/propidium iodide (PI) staining for phosphatidylserine externalization and JC-1 staining for mitochondrial membrane potential (MMP). The results were compared with conventional semen paramet...

the aim of this study was to evaluate changes that occur in motility parameters of progesterone treated mouse spermatozoa during course of hyperactivation. mouse spermatozoa treated with different doses of progesterone were videotaped after 10 min and 90 min of incubation. for each sperm, one second of movement of the head-midpiece junction was traced from the videotape and for each tracing sev...

introduction: to evaluate the quick cryopreservation technique on parameters of motility, morphology, and vitality of spermatozoa from normozoospermic ejaculates. materials and methods: in this prospective study, a total of 37 normal ejaculates were evaluated according to who criteria, then each sample was divided into two aliquets and sample was cryopreserved with quick freezing method. sperm ...

Fresh and post-thaw parameters (motility, morphology and viability) of stallion epididymal spermatozoa that have been and have not been exposed to seminal plasma were evaluated, and directly compared to fresh and post-thaw parameters of ejaculated spermatozoa. Six sperm categories of each stallion (n=4) were evaluated for motility, morphology and viability. These categories were fresh ejaculate...

BACKGROUND Reactive oxygen species (ROS)-mediated damage to human spermatozoa has been implicated in the pathogenesis of male infertility. Although ROS production by human spermatozoa has been extensively studied, the cell-to-cell variation in ROS production by spermatozoa at different stages of maturation has never been investigated. METHODS In this study, we determined ROS production by sub...

The aim of this study was to evaluate changes that occur in motility parameters of progesterone treated mouse spermatozoa during course of hyperactivation. Mouse spermatozoa treated with different doses of progesterone were videotaped after 10 min and 90 min of incubation. For each sperm, one second of movement of the head-midpiece junction was traced from the videotape and for each tracing sev...

background: sperm selection is generally used to describe methods for separation of spermatozoa for in-vitro-assisted reproduction. few studies reported relation in vivo fertility and semen parameters after selection of fertile spermatozoa; hence, our research attempt to assess different semen parameters after post-thaw (pt) or after selecting sperm sample (ssp), using density gradient separati...