this study aimed at determining the physico-morphological attributes, in vitrofertilityand their interrelationships in semen of sahiwal bulls. semen samples werecollected weekly for 6 weeks from ten mature sahiwal bulls divided randomly into 5 groups. the mean (±sem) of ejaculate volume (ml), mass activity (score 0-5), progressive sperm motility (%), sperm concentration (106/ml), live sperm (%)...

OBJECTIVE Several methods for evaluating semen quality have been developed in addition to traditional semen analysis. Most of these methods are very complex and require expensive technical equipment as well as special knowledge to be performed. The aim of this study was to establish an easy and cost-effective sperm penetration test for bovine semen using artificial media. Additionally a standar...

This study aimed at determining the physico-morphological attributes, in vitrofertilityand their interrelationships in semen of Sahiwal bulls. Semen samples werecollected weekly for 6 weeks from ten mature Sahiwal bulls divided randomly into 5 groups. The mean (±SEM) of ejaculate volume (mL), mass activity (score 0-5), progressive sperm motility (%), sperm concentration (106/mL), live sperm (%)...

Effect of supplementation of ascorbic acid, caffeine and chloroquine diphosphate in the dilutor on post-thaw quality of spermatozoa was studied. Thirty-two semen ejaculates (8 each from 2 Holstein Friesian and 2 crossbred; 50% Friesian x 50% Haryana) were studied. Ascorbic acid, caffeine and chloroquine diphosphate were added in Tris-egg yolk-glycerol (control) extender at the concentration of ...

The aim of this study was to evaluate the effect of sperm cryopreservation and the maturation state of the oocyte on the time course of canine gamete interaction during co-culture for periods of 1-10 h. Semen samples were obtained by digital stimulation and ejaculates processed as fresh, chilled and frozen samples. Sperm were co-cultured with immature or in vitro mature bitch oocytes for up to ...

In porcine oocytes, the function of the zona pellucida (ZP) with regard to sperm penetration or prevention of polyspermy is not well understood. In the present study, we investigated the effects of the ZP on sperm penetration during in vitro fertilization (IVF). We collected in vitro-matured oocytes with a first polar body (ZP+ oocytes). Some of them were freed from the ZP (ZP- oocytes) by two ...

Fertilization in the mouse is initiated by sperm beta 1,4-galactosyltransferase (GalTase) binding to terminal N-acetylglucosamine residues on the zona pellucida glycoprotein ZP3. Binding of ZP3 induces exocytosis of the sperm acrosome, whose contents are believed to digest a penetration slit in the zona matrix through which sperm reach the egg. As a consequence of acrosomal exocytosis, GalTase ...

Electroejaculate traits and circulating follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone concentrations were analyzed in adult leopard cats (Felis bengalensis), a rare felid species indigenous to east Asia. The ability of leopard cat sperm to bind and penetrate zona-free hamster ova and zona-intact domestic cat oocytes in vitro was examined as a means of testing sp...

The presence of white blood cells in semen has been associated with male infertility. Previous studies indicate that pyospermia occurs in conjunction with decreases in sperm motility, number of normal sperm forms, and penetration rates in the zona-free hamster egg sperm penetration assay. We have evaluated the relationship of seminal white blood cells and sperm function, as reflected in the zon...

Fifteen monoclonal antibodies (MAbs) raised against human sperm cells were evaluated for reactions against human sperm by indirect immunofluorescence, immunocytochemistry, agglutination, complement-dependent immobilization, cervical mucus penetration, and hamster egg penetration assays. The MAbs were analyzed for specificity by immunofluorescent reactions with peripheral blood lymphocytes and s...