background: this case-control study was designed with the aim of evaluating the role of sperm, oxidative stress and dna damage in idiopathic recurrent pregnancy loss (irpl). this pilot study is the first study done on the indian population which reports the association between dfi, tac and ros in couples experiencing irsa. methods: twenty infertile men with a history of irpl and 20 fertile cont...

evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear dna integrity of spermatozoa. it has been suggested that altered nuclear chromatin struc...

Evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. Routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin struc...

introduction: the novel development of the (hba) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. the purpose of this study was to evaluate the correlation between hba score and histone deficiency, protamine content and dna fragmentation. methods: during april- march 2012 patients who were enrolled at shiraz-...

Background: Sperm chromatin integrity assessment has been suggested as fertility predictor. The aim of this study was to examine the relationship between the results of sperm chromatin structure assay (SCSA) and the outcome of intrauterine insemination IUI. Materials and Methods: A total of 576 consecutive couples undergoing IUI were included and IUI was performed. SCSA results were expressed a...

sperm is produced by the testis and mature in the epididymis. for having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. the effects of genetic and environmental factors result in sperm vulnerability to damage in the process of spermatogenesis and maturation. in recent years, the fe...

It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay, the comet assay, the sperm chromatin structure assay (SCSA) and the sperm chromatin d...

Abbreviations: AI: Artificial Insemination; BCF: Beat Cross Frequency; PI: Propidiumiodide; ZBA: Zona Binding Assay; DFI: DNA Fragmentation Index; HZA: Hemizona Assay; SCSA: Sperm Chromatin Structure Assay; AOT: Acridine Orange Test; SCD: Sperm Chromatin Dispersion Test; ROS: Reactive Oxygen Species; VSL: Straight Line Velocity; LIN: linearity; VCL: Curvilinear Velocity; CTC: Fluorescent Chlort...

Background: Globozoospermia is an uncommon disorder of sperm morphology associated with severe male infertility. Patients who contain both normal and round-headed sperm are called partial globozoospermic. It is possible that round-headed sperm may have an abnormal chromatin structure and /or DNA strand breaks. Moreover, sperm DNA fragmentation and protamine deficiency is shown to be associated ...