DEVELOPMENTAL CONTEXT OF SPERM CELLS ........................................ 190 Structural Characterization ..................................................................... 190 Sperm Dimorphism and Preferential Fertilization .......................................... 191 ISOLATION OF SPERM CELLS ................................................................. 192 Technical Details and P...

shape and movement features of sperms are important parameters for infertility study and treatment. in this article, a new method is introduced for characterizing sperms in microscopic videos. in this method, first a hypothesis framework is defined to distinguish sperms from other particles in captured video. then decision about each hypothesis is done in following steps: selecting some primary...

Detection of sperm-immobilizing antibodies in women may have relevance for diagnosis of immunological infertility. Infertile women in whom sperm-immobilizing antibodies are detected can be refractory to conventional treatments such as timed intercourse or intrauterine insemination (IUI) because the antibodies secreted in the female reproductive tract might impair sperm passage, inhibit fertiliz...

Mammalian spermatozoa, particularly those of rodent species, are extremely complex cells and differ greatly in form and dimensions. Thus, characterization of sperm size and, particularly, sperm shape represents a major challenge. No consensus exists on a method to objectively assess size and shape of spermatozoa. In this study we apply the principles of geometric morphometrics to analyze rodent...

The identification and characterization of germ cell-specific genes are essential if we hope to comprehensively understand the mechanisms of spermatogenesis and fertilization. Here, we searched the mouse UniGene databases and identified 13 novel genes as being putatively testis-specific or -predominant. Our in silico and in vitro analyses revealed that the expressions of these genes are testis-...

Molecular characterization of sperm proteins and their immunoreactions to sera from subfertile/infertile animals may give deeper insight to the immunological reason of infertility. The purpose of this study was to characterize antigenic sperm proteins by reacting with blood serum and cervical mucus of variably inseminated heifers/cows using immunoblotting and immunofluorescence techniques. Immu...

Qualitative and quantitative analysis of porcine sperm transcripts and characterization of a normalized cDNA library Thesis for the attainment of the title Doctor in Veterinary Medicine

1. O’Rand MG, Widgren EE, Fisher SJ. Characterization of the rabbit sperm membrane autoantigen, RSA, as a lectin-like zona binding protein. Dev Biol. 1988;129:231-240. 2. Lim SH, Wang Z, Chiriva-Internati M, Xue Y. Sperm protein 17 is a novel cancer-testis antigen in multiple myeloma. Blood. 2001;97:1508-1510. 3. Chiriva-Internati M, Wang Z, Salati E, Timmins P, Lim SH. Tumor vaccine for ovaria...

Several studies exhibit the presence of Ricinus Communis Agglutinin I (RCA) binding glycocalyx in mammalian spermatozoa. However, the molecular characterization of RCA binding glycocalyx in sperm membranes and its mechanism of action are poorly understood. The objective of the study was to identify and to characterize RCA binding glycoprotein of the bovine sperm plasma membranes (PM). Lectin bl...