In a previous publication (1) from this laboratory, it was demonstrated that the transamination of amino acids to a-ketoglutaric acid is a reaction of wide scope. Although the data reported in the earlier paper permitted the computation of Q transaminase values, these values were of limited quantitative significance since no attempt was made to study the kinetics of these reaction systems, the ...

Focus of this paper is the description of common used methods for hexavalent chromium measurement. This work is focused especially on commonly available spectrophotometric method based on the reaction with 1,5-diphenylcarbazide, because not every laboratory is equipped with modern devices for this pollutant measurement,. Special attention was focused on the variability possibilities of the basi...

− A spectrophotometric analyser of food (SAF) is a spectrophotometric instrument dedicated to measuring physical and/or chemical parameters characterizing a predefined class of chemical or biochemical substances which appear in food raw materials or products. It is usually composed of a spectrophotometric transducer and a digital processor. The latter is transforming raw measurement data, provi...

A spectrophotometric recycling assay for the quantitation of L-phenylalanine (and phenylpyruvate) has previously been reported (Cooper et al., Anal. Biochem. 183, 210-214, 1989). The procedure involves the coupling of bacterial phenylalanine dehydrogenase with rat kidney cytosolic glutamine transaminase K. The latter enzyme possesses high affinity for phenylpyruvate. Recycling results in a > or...

A simple, reliable and rapid method for the spectrophotometric determina­ tion of carboxyhemoglobin in fresh blood is described. A blood hemolysate is prepared by diluting a blood sample with 0.4 percent NH4 OH. Addition of sodium dithionite deoxygenates hemoglobin (and reduces methemoglobin if present) without affecting carboxyhemoglobin. Comparison of the 541/555 nm absorbance ratio for the h...

A rapid and sensitive direct spectrophotometric method for the determination of cerebrospinal fluid protein which usesas little as 0.1 ml. of sample has been described. The result is not affected by the ratio of albumin to globulin in the sample. Interfering substances are removed prior to spectrophotometric measurement with an automatic separation chamber employing the principle of gel filtrat...

a simple, sensitive and accurate method for spectrophotometric determination of dalacinphosphatehas been established. the method is based on the reduction of atomic absorptioncalcium ion by phosphate. because, there is a phosphate mole in every dalacin mole structureand with measurement of phosphate, dalacin is beyond measurement too. therefore, the reactionbetween acidic hydrolysis products of...

A simple, sensitive and accurate method for spectrophotometric determination of Dalacinphosphatehas been established. The method is based on the reduction of atomic absorptioncalcium ion by phosphate. Because, there is a phosphate mole in every Dalacin mole structureand with measurement of phosphate, Dalacin is beyond measurement too. Therefore, the reactionbetween acidic hydrolysis products of...