abstract- desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. a pcr-based method was developed for the identification of 3 major desert truffles of iran: terfezia claveryi, tirmania pinoyi and tirmania nivea based on internal transcribed spacers of rdna. two specific pcr primers were designed for t. claveryi, 4 for t. pinoyi, and 2 for ...

Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication.  Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward primer. In the second multiple...

an experiment was conducted, using two year old kinnow plants, budded on rough lemon rootstock, inoculated with different isolates of amf fungi viz., glomus manihotis, gigaspora gigantean and glomus mosseae singly as well as in mixed culture. five primers vans1, ns61,ns21,vaglo,vagiga specific to glomus and gigaspora species were designed and used in a polymerase chain reaction with dna extract...

to evaluate allelic diversity at loci encoding low molecular weight glutenin subunits (lmw-gs) among 62 genotypes of bread wheat, nine specific primer pairs for glu-a3, glu-b3, glu-d3 loci were employed. pcr products were separated on 2% agarose gels. two pairs of specific primers, at amplified glu-a3 locus, 8 alleles were detected the c allele of which was the most abundant with a frequency of...

Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni. Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was sele...