Salmonella outer protein D (SopD) is a type III secreted virulence effector protein from Salmonella enterica. Full-length SopD and SopD lacking 16 amino acids at the N-terminus (SopDDeltaN) have been expressed as fusions with GST in Escherichia coli, purified with a typical yield of 20-30 mg per litre of cell culture and crystallized. Biophysical characterization has been carried out mainly on ...

SgrS RNA is a model for the large class of Hfq-associated small RNAs that act to posttranscriptionally regulate bacterial mRNAs. The function of SgrS is well-characterized in nonpathogenic Escherichia coli, where it was originally shown to counteract glucose-phosphate stress by acting as a repressor of the ptsG mRNA, which encodes the major glucose transporter. We have discovered additional Sgr...

background: despite of the advances in infectious diseases prevention and food technology, food-borne diseases are considered major problems in developed and developing countries. meat plays a key role in transferring zoonotic diseases to human. objectives: this study was conducted in south of tehran, iran, to investigate the prevalence rate of salmonella spp. in packed and unpacked red meat an...

Salmonella enterica serotype Typhi is a strictly human adapted pathogen that does not cause disease in nonprimate vertebrate hosts, while Salmonella enterica serotype Typhimurium is a broad-host-range pathogen. Serotype Typhi lacks some of the proteins (effectors) exported by the invasion-associated type III secretion system that are required by serotype Typhimurium for eliciting fluid secretio...

Sequence characterized amplified region (SCAR) markers are one of the most effective and accurate tools for microbial identification. In this study, we applied SCAR markers for the rapid and accurate detection of Phytophthora katsurae, the casual agent of chestnut ink disease in Korea. In this study, we developed seven SCAR markers specific to P. katsurae using random amplified polymorphic DNA ...

Salmonella resides within host cells in a vacuole that it modifies through the action of virulence proteins called effectors. Here we examined the role of two related effectors, SopD and SopD2, in Salmonella pathogenesis. Salmonella enterica serovar Typhimurium (S. Typhimurium) mutants lacking either sopD or sopD2 were attenuated for replication in the spleens of infected mice when competed aga...

OBJECTIVES To assess the legibility and completeness of handwritten prescriptions and compare with electronic prescription system for medication errors. DESIGN Prospective study. SETTING King Khalid University Hospital (KKUH), Riyadh, Saudi Arabia. SUBJECTS AND METHODS Handwritten prescriptions were received from clinical units of Medicine Outpatient Department (MOPD), Primary Care Clinic...

Abstract Salmonella utilizes translocated virulence proteins (termed effectors) to promote host cell invasion. The effector SopD contributes invasion by promoting scission of the plasma membrane, generating -containing vacuoles. is expressed in all lineages and plays important roles animal models infection, but its targets are unknown. Here we show that can bind inhibit small GTPase Rab10, thro...

OBJECTIVE The aim of this study was to evaluate the relative error (RE) in the thyroid absorbed dose (TD) of iodine-131 (131I) in patients with Graves' disease comparing the simplified Quimby-Marinelli-Hine formula method (sQMHF) and the Standard Operational Procedures for dosimetry (SOPD) recommended by the European Association of Nuclear Medicine. PATIENTS AND METHODS This study included 45...

Salmonella can survive for long periods under extreme desiccation conditions. This stress tolerance poses a risk for food safety, but relatively little is known about the molecular and cellular regulation of this adaptation mechanism. To determine the genetic components involved in Salmonella's cellular response to desiccation, we performed a global transcriptomic analysis comparing S. enterica...