the liver fluke fasciola hepatica causes fascioliasis, a liver disease in most part of the world and particularly in north of iran. diagnosis of the diseases is anchored in coprological manner but serological methods are preferable due to some obscurities. in this study, sera obtained from human patients infected with fasciola hepatica were tested by the enzymelinked immunotrotransfer blot (eit...

human visceral and ocular toxocariasis can occasionally be a serious or life-threatening condition, especially for children. the diagnosis and confirmation of the disease mostly based upon serological tests. the purpose of the present study was to evaluate somatic antigen with regard to its application in a routine diagnostic laboratory test. using gel-filtration chromatography, fraction no.1 o...

background: ornithobilharziosis as one of the parasitic infections may give rise to serious economic problems in animal husbandry. the aim of the study was to prepare and compare the somatic and excretory-secretory (es) antigens of o. tur­kestanicum in gel diffusion test. methods: excretory-secretory (es) and somatic antigens of ornithobilharzia turkestanicum were prepared from collected worms ...

Johnson, E. M. (Walter Reed Army Institute of Research, Washington, D.C.), Barbara Krauskopf, and L. S. Baron. Genetic mapping of Vi and somatic antigenic determinants in Salmonella. J. Bacteriol. 90:302-308. 1965.-The Vi antigen and somatic antigen 9 were transferred to Salmonella typhimurium recipients by mating with S. typhosa Hfr TD-7, and the genetic determinants of these antigens were loc...

Somatic hypermutation and clonal selection lead to B cells expressing high-affinity antibodies. Here we show that somatic mutations not only play a critical role in antigen binding, they also affect the thermodynamic stability of the antibody molecule. Somatic mutations directly involved in antigen recognition by antibody 93F3, which binds a relatively small hapten, reduce the melting temperatu...

Due to its role in human Trichinellosis, there are increasing global requirements for reliable diagnostic method for Trichinella spiralis infection in pigs. Consequently, there is a need for selection of potent diagnostic antigen that could be successfully utilized in the diagnosis. Currently, two antigens (somatic and excretory-secretory antigens of T. spiralis larvae) were evaluated for the d...

Canine angiostrongylosis, caused by the nematode Angiostrongylus vasorum, is an emerging cardiopulmonary disease in Europe which can be fatal if left untreated. We determined the diagnostic value of the specific detection of antibodies against A. vasorum adult somatic antigen, adult excretory/secretory (E/S) antigen and first stage larvae (L1) somatic antigen in ELISAs. Also, A. vasorum adult s...

Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. Therefore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for serological identification bovine ephemeral fever (BEF)-infected animals. The aim of this ...