conclusions the rabies virus np could be extracted and purified in an appropriate amount from infected cell culture. the results of sds-page analysis showed that the intact rabies virus np had been purified properly and thus could be used for further steps to produce the specific diagnostic rabies conjugate. results the volume of the lysate was 15 ml and it became 2.5 ml after purification, wit...

preparation of chitosan (cs) microspheres as a novel drug delivery vehicle for intranasal immunization using high, medium and low cs molecular weight (mw) was investigated in this study. diphtheria toxoid (dt) was used as a model antigen. the emulsion-solidification method was adopted for microencapsulation of dt.  in the first step, following the purification of semi-crude dt by the ion-exchan...

introduction: discharge of textile wastewater causes the reduction of sunlight penetration, interferes with the receiving waters ecology and damage the environment. the aim of this study was to determine the removal efficiency of reactive blue 19 dye from aqueous solutions by cuttlefish bone powder. materials & methods: this study was performed experimentally and in laboratory scale. in this re...

The toxicity and corrosion potential of hydrogen sulfide in raw biogas underlines the need for biogas purification. Several techniques available for removal of hydrogen sulfide from biogas are out of the reach for common end users due to lack of knowledge, higher running costs, and insufficient operational skills. The present experimental study aims to propagate hydrogen sulfide removal techniq...

Homeobox genes encode transcription factors which play important roles in the developmental processes of many multicellular organisms. TGIFLX/Y (TGIFLX and TGIFLY) are members of the homeobox superfamily of genes. Their expressions are specifically detected in the human adult testis but their functions are remained to be investigated. In this investigation we cloned full length of TGIFLY cDNA a...

background: alkyl hydroperoxide reductase (ahpc) of helicobacter pylori is considered as a diagnostic antigen. there­fore, this antigen can be used to detect h. pylori infection by stool immunoassays such as elisa. the aim of this study was to simplify the ahpc protein purification procedures. methods: for whole cell protein extraction, the bacterial cells were ruptured by octly-β-d glucopyrano...

recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...