Soils have a great potential to accumulate carbon from atmospheric sources, but we possess few quantitative tools to predict and understand the conversion of C from plant sources into stable soil organic matter. Evaluation of present methods used for analyzing the major form of carbon present in plants, carbohydrates, found that development of ‘total carbohydrate’ methods were originally based ...

A 345-bp gene that encodes human bone morphogenetic protein-2 (hBMP-2) has been synthesized. The codon usage of the resulting gene was modified to include those triplets that are utilized in highly expressed Escherichia coli genes. The hBMP-2 gene was efficiently expressed in E. coli as a soluble and active protein. Since the recombinant hBMP-2 was readily solublized, no further solublization s...

the rhizosphere is the area around the root of a plant occupied by a unique population of usefulbacteria known as plant growth promoting rhizobacteria (pgpr). in this study, the isolation andidentification of rhizobacteria from orange (citrus sinensis) orchards using 16s rrna gene, as well asbiological and biochemical assays is reported. analysis of 16s rrna gene was confirmed bybiological and ...

Purpose: Hydrotropes increases the solubility of organics in water. Objective of present investigation was to enhance the solubility of griseofulvin using the technique of hydrotropic solubilization technique and convert them into suitable oral liquid dosage form (suspension) useful for enhancement of bioavailability. Methods: 0.5M, 1M, 2M of the hydrotropes (tri sodium citrate, urea, sodium ac...

collagen, which is widely distributed in pluricellular animals, is one of the most fundamental constituents of the extracellular matrix, and plays mechanically or physiologically important roles in their bodies. in this study, the biochemical and physical characteristics of pepsin-solubilized collagen from the mantle of yesso scallop (ympc), a by-product of processing, was determined. electroph...

       Purpose of this study was to investigate the effect of the presence of the water soluble polymers viz HPMC, PVP and PEG 6000 on aqueous solubility and complexation abilities of felodipine with or without presence of β-cyclodextrin (βCD) and HPβCD by phase solubility studies. The data revealed the initial increase in drug solubility followed by plateau in t...

Expression of the leaderless pac gene (LL pac), which lacks the coding region for the signal peptide of penicillin acylase (PAC), in Escherichia coli was conducted. It was demonstrated that the PAC precursor, proPAC, can be produced and even processed to form mature PAC in the cytoplasm, indicating that the posttranslational processing steps for PAC maturation can occur in both the periplasm an...

The glycoside hydrolase family 9 cellulase (Cel9) from Clostridium phytofermentans has a multi-modular structure and is essential for cellulose hydrolysis. In order to facilitate production and purification of Cel9, recombinant Cel9 was functionally expressed in Escherichia coli. Cel9 exhibited maximum activity at pH 6.5 and 65 degrees C on carboxymethyl cellulose in a 10-min reaction period. T...

BACKGROUND Paclitaxel (taxol) is a very effective anticancer drug. However, the solution used to enhance the water solubility of paclitaxel, a mixture of polyoxyethylene castor oil (Cremphor EL) and dehydrated ethanol, causes irritation. Our aim is to develop a water-soluble taxol with a high anticancer effect. MATERIALS AND METHODS Taxol derivatives (conjugation with aminated dextran, Dex-TX...