delayed-type hypersensitivity reaction measured by skin testing, is an important tool for evaluation of cellular immune response in leishmaniasis and has been used recently as an indication of exposure to infection. skin testing in leishmania infection needs a standard reagent with high specificity, sen sitivity, and potency. in the present work, a soluble leishmanin, and three whole parasite p...

Delayed-type hypersensitivity reaction measured by skin testing, is an important tool for evaluation of cellular immune response in leishmaniasis and has been used recently as an indication of exposure to infection. Skin testing in leishmania infection needs a standard reagent with high specificity, sen‌sitivity, and potency. In the present work, a soluble leishmanin, and three whole parasite p...

Cutaneous leishmaniasis (CL) is an endemic disease in some parts of Iran and it has high morbidity in some areas of the country. The disease is detected by parasitological examinations including direct microscopic and culture tests. This comparative study aimed to evaluate the relationship between positivity of the leishmanin skin test (LST), microscopically examination and clinical forms of CL...

OBJECTIVE To test the safety and immunogenicity of two doses of autoclaved L. major (ALM) vaccine mixed with BCG. SETTING Kala-azar endemic area of eastern Sudan. DESIGN This was a randomised, double blind and BCG controlled phase I/II study. SUBJECTS Eighty healthy volunteers (forty children and forty adults) with no past history of kala-azar, no reactivity to leishmanin antigen and with...

Sixty-five patients were diagnosed with visceral leishmaniasis (VL) on Margarita Island in the decade from 1990 to1999; 86.2% were <= 3 years old. All were leishmanin-negative at diagnosis. Evaluation of 23 cured patients in 1999 revealed that 22/23 had converted to leishmanin-positive; five had persisting antibodies to rK39 antigen, with no clinical evidence of disease. Leishmanin tests were p...

Since the early 1990s this view changed with the recognition of autochthonous cutaneous leishmaniasis. The first documented case in 1992 was a young man from Ambalantota who initially gave a history of contact with dry zone jungle but later denied this history (1). This was followed a few years later by a number of cases from different parts of the island. The second and third case reports were...

The aim of this study was to identify splenic immuno-inflammatory patterns associated with natural infection by Leishmania chagasi. Spleen samples were obtained from 72 stray dogs from an endemic area of visceral leishmaniasis. The animals were grouped into four categories as follows: (i) potentially resistant to visceral leishmaniasis, with a positive leishmanin skin test result, and negative ...

cutaneous leishmaniasis (cl) is an endemic disease in some parts of iran and it has high morbidity in some areas of the country. the disease is detected by parasitological examinations including direct microscopic and culture tests. this comparative study aimed to evaluate the relationship between positivity of the leishmanin skin test (lst), microscopically examination and clinical forms of cl...

Associations among parameters commonly used as markers of infection by Leishmania sp., or of susceptibility to visceral leishmaniasis, were investigated in 325 stray dogs from an area where this disease is endemic. Evidence of infection (presence of Leishmania in splenic cultures, positive leishmanin skin test (LST) or detection of anti-Leishmania antibody activity in the serum) was found in 57...