background: splicing by overlap extension (soe) pcr is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function.objectives: we introduced a nested-soe-pcr (n –soe-pcr) in order to increase the specificity and generating mutations in a gene by soe-pcr.  materials and methods: genomic dna from bacillus thermoca...

BACKGROUND Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein's structure and function. OBJECTIVES We introduced a nested-SOE-PCR (N -SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR. MATERIALS AND METHODS Genomic DNA from Bacillus thermoc...

Recently, State of energy (SOE) has become one of the most fundamental parameters for battery management systems in electric vehicles. However, current information is critical in SOE estimation and current sensor is usually utilized to obtain the latest current information. However, if the current sensor fails, the SOE estimation may be confronted with large error. Therefore, this paper attempt...

A method for automatic generation of syntax-oriented editors (SOE) for high level programming languages is presented. It is based on a special template definition metalanguage. The SOE functional environment including the operations with source files and internal representation of the programs in form of abstract syntax tree was implemented as an independent modular structure. As a result of ta...

Service Oriented Enterprise (SOE) has brought up requirement for performance measurement of the novel business model in its dynamic and flexible business environment. To solve the problem, conception of SOE is discussed from business perspective firstly, and an operation architecture of SOE is proposed and analyzed. The Service Oriented Economic Value Added (SO-EVA) performance measurement mode...

Background: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. Objectives: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR.   Materials and Methods: Genomic DNA from Bacillus thermo...

PURPOSE To evaluate the function of the blood aqueous barrier(BAB) in the eyes with silicone oil emulsification (SOE). METHODS Protein concentrations, expressed in albumin equivalents,were determined in aqueous humor of the eyes with SOE in 11 consecutive patients by means of proton nuclear magnetic resonance (1H NMR) spectroscopy. Correlations with various clinical factors were studied. RE...

In this paper, we examine the role of political connections by analyzing the short-and long-term performance of Chinese state owned enterprise (SOE) acquirers relative to private owned enterprise (POE) from 1994 to 2008. The empirical result shows that 1) SOE acquirers outperform the POE acquirer both in terms of long-run stock and operating performance; 2) announcement effect of POE acquirer t...

This study aims to investigate the anti-inflammatory responses and mechanisms of Siegesbeckia orientalis ethanol extract (SOE). In cell culture experiments, RAW264.7 cells were pretreated with SOE and stimulated with lipopolysaccharide (LPS) for inflammatory mediators assay. In animal experiments, mice were tube-fed with SOE for 1 week, and s.c. injected with λ-carrageenan or i.p. injected with...

Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. In the SCNT experiment, nuclear reprogramming is induced by exposure of introduced donor nuclei to the recipient cytoplasm of matured oocytes. However, because the efficiency of SCNT still remains low, a combination of SCNT technique with the ex-ov...