Heparin was cleaved with nitrous acid at pH 1.5 and the products were reduced with Na+ boro[3H]hydride to generate a mixture of di- and tetrasaccharides having anhydro-D-[3H]mannitol (AManR) residues on their reducing terminals. The products were purified to homogeneity by gel filtration and high-performance liquid chromatography. For each oligosaccharide, the proportions of D-glucuronic acid (...

Pelis, Ryan M., and J. Larry Renfro. Role of tubular secretion and carbonic anhydrase in vertebrate renal sulfate excretion. Am J Physiol Regul Integr Comp Physiol 287: R491–R501, 2004; 10.1152/ajpregu.00084.2004.—The renal proximal tubule of vertebrates performs an essential role in controlling plasma SO4 2 concentration ([SO4 2 ]). Although net tubular SO4 2 reabsorption is the predominate co...

One of the modes of action of the red blood cell anion transport protein is the electrically silent net exchange of 1 Cl- for 1 SO4= and 1 H+. Net SO4(=)-Cl- exchange is accelerated by low pH or by conversion of the side chain of glutamate 681 into an alcohol by treatment of intact cells with Woodward's reagent K (WRK) and BH4-. The studies described here were performed to characterize the elec...

Cosmogenic sulfur-35 in water as dissolved sulfate ((35)SO4) has successfully been used as an intrinsic hydrologic tracer in low-SO4, high-elevation basins. Its application in environmental waters containing high SO4 concentrations has been limited because only small amounts of SO4 can be analyzed using current liquid scintillation counting (LSC) techniques. We present a new analytical method f...

[1] Biogeochemical cycling of N and S was examined at two watersheds in the Adirondack Mountains, New York, to better understand the retention and loss of these elements during winter and spring snowmelt. We analyzed stable isotope compositions of NO3 (dN-NO3 , dO-NO3 ) and SO4 2 (dS-SO4 2 , dO-SO4 2 ), along with concentrations and fluxes of NO3 and SO4 2 , in precipitation, throughfall, snowp...

A year-long field study to characterize the ionic species in PM2.5 was carried out in Shanghai and Beijing, China, in 1999–2000. Weekly samples of PM2.5 were collected using a special low flow rate (0.4 lmin ) sampler. In Shanghai, SO4 2 NO3 and NH4 + were the dominant ionic species, which accounted for 46%, 18% and 17% of the total mass of ions, respectively. Local SO2 emissions were an import...

Most vertebrates, including cartilaginous fishes, maintain their plasma SO4 (2-) concentration ([SO4 (2-)]) within a narrow range of 0.2-1 mM. As seawater has a [SO4 (2-)] about 40 times higher than that of the plasma, SO4 (2-) excretion is the major role of kidneys in marine teleost fishes. It has been suggested that cartilaginous fishes also excrete excess SO4 (2-) via the kidney. However, li...

Sulfate reduction and SRB growth kinetics were studied in growth media for SRB (Dev et al., 2014), MSRB containing marine waste extract as nitrogen source with increasing SO4 concentration, such as 1500, 3500, 5500 and 7500 mg L. With increase in SO4 concentration, SO4 reduction rate, its efficiency, SRB specific growth rate, HS generation rate were deteriorated. In the presence of 1500 mg L SO...

Sulfate (SO4(2-)) concentrations and fluxes were studied for multiple storm events in the Point Peter Brook watershed, a glaciated, forested watershed located in Western New York, USA. Investigations were performed across one large (696 ha) and three small (1.6-3.4 ha) catchments with varying extent of riparian and wetland areas. Concentrations of SO4(2-) in groundwater sources (mean values: 23...

Bovine aortic smooth-muscle cells, bovine aortic endothelial cells, and IMR-90 human embryonic lung fibroblasts were tested to determine their ability to use cysteine or cysteine metabolites as a source of sulphate (SO4). Cells were incubated in SO4-depleted medium containing [3H]glucosamine plus 0.2 mM-cystine, 0.3 mM-cysteinesulphinic acid or 0.3 mM-sulphite (SO3). The [3H]chondroitin sulphat...