نتایج جستجو برای: small interfering rna

تعداد نتایج: 1014115  

Journal: :genetics in the 3rd millennium 0
الیکا اسماعیل زاده elika esmaeilzadeh مهدی بنان mehdi banan دکترای ژنتیک مولکولی، استادیارمرکز تحقیقات ژنتیک

in this study, several designed creb1-specific shrnas cloned into lentiviral transfer vectors were screened in293t by transient transfection assays. relative creb1 expression was determined by relative qrt-pcr. all of the designed shrnas gave some level of knockdown. one shrna was more effective for knocking down the specific gene among others. the predicted sirnas activities as determined by r...

Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...

Journal: :Journal of Investigative Dermatology 2013

Journal: :iranian red crescent medical journal 0
moslem jafari sani biochemistry department, school of medicine, shahroud university of medical sciences, shahroud, ir iran foad yazdi biotechnology department, faculty of medicine, tehran university of medical sciences, tehran, ir iran masoomeh masoomi karimi immunology department, school of medicine, torbat heydariyeh university of medical sciences, torbat heydariyeh, ir iran javad alizadeh department of molecular medicine, biotechnology research center, pasteur institute of iran, tehran, ir iran majid rahmati biotechnology department, faculty of medicine, tehran university of medical sciences, tehran, ir iran ali zarei mahmudabadi biochemical department, chemical injuries research center, baqiyatallah university of medical sciences, tehran, ir iran; biochemical department, chemical injuries research center, baqiyatallah university of medical sciences, tehran, ir iran. tel: +98-9123277532, fax: +98-2122830262

background angiogenesis is an important biological process involved in the proliferation of endothelial cells, tumor growth and metastasis. vascular endothelial growth factor (vegf) is considered as a prominent regulator of angiogenesis which exerts the aforementioned effect(s) through its respective receptors (vegfr1 and vegfr2). vegf receptors are targeted as a therapeutic candidate for cance...

ژورنال: دانشور پزشکی 2009

 Introduction: RNA interference (RNAi) is a phenomenon of gene silencing that uses double-stranded RNA (dsRNA), specifically inhibits gene expression by degrading mRNA efficiently. The mediators of degradation are 21- to 23-nt small interfering RNAs (siRNA). The use of siRNAs as inhibitors of gene expression has been shown to be an effective way of studying gene function in mammalian cells.  Ai...

Journal: :Nucleic Acid Therapeutics 2011

Journal: :Fundamental & Clinical Pharmacology 2009

Journal: :Proceedings of the National Academy of Sciences 2003

Mozhgan Rasti, Rita Arabsolghar

Background: Electroporation is a valuable tool for small interfering RNA (siRNA) delivery into cells because it efficiently transforms a wide variety of cell types. Since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect MDA-MB 468 human breast cancer cell with siRNA. ...

Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning stra...

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