Objective: Overexpression of SLUG has been implicated in promoting migration and invasion of many cancer cells, including anaplastic thyroid cancer (ATC) cells. Thus, targeted down-regulation of SLUG expression in ATC cells and determination of its implications would provide new treatment approaches for disease management. Methods: Small interfering RNA (siRNA) targeting SLUG (SLUG siRNA) was u...

Slug is an E-cadherin repressor and a suppressor of PUMA (p53 upregulated modulator of apoptosis) and it has recently been demonstrated that Slug plays an important role in controlling apoptosis. In this study, we examined whether Slug's ability to silence expression suppresses the growth of cholangiocarcinoma cells and/or sensitizes cholangiocarcinoma cells to chemotherapeutic agents through i...

BACKGROUND Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive carcinomas of the gastrointestinal tract. We assessed the relevance of Slug in measuring the invasive potential of ESCC cells in vitro and in vivo in immunodeficient mice. METHODS We utilized RNA interference to knockdown Slug gene expression, and effects on survival and invasive carcinoma were evaluated using ...

BACKGROUND Slug has been found to promote migration and invasion of many cancer cells, including anaplastic thyroid cancer (ATC). Thus, targeting Slug expression could provide new approaches for the treatment of patients with ATC. MATERIAL AND METHODS Small interfering RNA (siRNA) targeting Slug (Slug siRNA) was used to construct clonal derivatives in the metastatic ATC SW1736 cells. Slug cDNA ...

PURPOSE TGFbeta is a potent candidate for epithelial-mesenchymal transition (EMT) during the development of anterior polar cataracts in the human lens. The Snail superfamily is involved in EMT through the repression of E-cadherin production. This study was conducted to determine whether the Snail gene family is activated in the process of TGFbeta1-induced EMT and how TGFbeta1 regulates the expr...

PURPOSE In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cancer cells. Here, we investigated the clinical significance of Slug in lung adenocarcinoma and the role of Slug in the process of cancer cell invasion and metastasis. EXPERIMENTAL DESIGN Real-time quantita...

slug (snai2), a regulator of epithelial-to-mesenchymal transition (emt), is a zinc finger transcription factor involved in neurulation. intriguingly, snai2 gene expression has been linked to the aggressiveness of neuroblastoma, melanoma and lung, colon, ovarian and breast carcinoma. of note, many metastatic cancers recapitulate emt to enhance cell motility and invasion. moreover, metastasis is ...

OBJECTIVES This study explored the expression and function of Slug in human extrahepatic hilar cholangiocarcinoma (EHC) to identify its role in tumor progression. METHODS The expression of Snail and Slug mRNA in 52 human tissue samples of EHC was investigated. The mRNA of Snail and Slug were quantified using reverse transcriptase-PCR, and correlations with E-cadherin expression and clinicopat...

OBJECTIVE Previous studies have confirmed Slug as a key player in regulating phenotypic changes in several cell models, however, its role in smooth muscle cells (SMC) has never been assessed. The purpose of this study was to evaluate the expression of Slug during the phenotypic switch of SMC in vitro and throughout the development of vascular remodeling. METHODS AND RESULTS Slug expression wa...

An epithelial-mesenchymal cell transformation occurs during the development of the endocardial cushions in the atrioventricular (AV) canal of the heart. We hypothesized that the transcription factor Slug is required for this epithelial-mesenchymal cell transformation since Slug is required for similar transformations during gastrulation and neural crest differentiation in chicken embryos. We fo...