the capability to preserve human oocytes, blastocysts, ovarian tissue and spermatozoa is an important tool in human assisted reproductive techniques. this preservation allows patients undergoing chemotherapy or radiotherapy to preserve their fertility, and helps to attain all benefits from the costly ovarian superovulation therapies prior to art. the primary goal in establishing an appropriate ...

in order to investigate the flexural behaviors of rc beams after freeze-thaw cycles, compressive strength test of concrete cubes after 0, 50, 100, 125 freeze-thaw cycles were made, and static flexural experiment of 48 rc beams after 0, 50, 100, 125 freeze-thaw cycles were made. the relationships of relative compressive strength, mass loss rate, relative dynamic elastic modulus and numbers of fr...

Preventing cartilage injury is important in minimizing the long term debilitating effects of osteoarthritis. Accurate subfracture injury prediction must take into account the possible effects that freeze thaw cycles may have on the mechanical properties of cartilage tissue. This paper addresses this concern with matched pair testing of various low temperature storage techniques against fresh co...

Decellularized arterial scaffolds have achieved success in advancing toward clinical use as vascular grafts. However, concerns remain regarding long-term preservation and sterilization of these scaffolds. Freeze drying offers a means of overcoming these concerns. In this study, we investigated the effects of various freeze-drying protocols on decellularized porcine carotid arteries and conseque...

The Arrhenius activation energy for water permeability, (DeltaE(a,H(2)O)) through stem cortical tissue of red osier dogwood (Cornus sericea L.) was determined after treatments which cause membrane rupture as well as after a lethal slow freeze and subsequent slow rewarming. The latter value was higher than the former, but was indistinguishable from the DeltaE(a,H(2)O) found for healthy tissue. I...

Cracks can form during the freeze-drying of freeze cast ceramic suspensions while attempting to produce dense ceramics. The contain alumina particles dispersed in cyclohexane. rate drying is controlled by pressure and temperature (slow at atmospheric −15°C fast under vacuum slowly increases from −80°C room temperature). X-Ray micro-computed tomography was used characterize internal crack format...

342 d o i : 10.84/ejd .208.0402 Most authors recommend a quick freeze and slow thaw method of tissue destruction. Slow cooling produces extracellular ice, but this is not as damaging as rapid cooling that produces intracellular ice formation. With slow thawing, an increased concentration of electrolytes and recrystallization occurs, which is also damaging to cells. Thus the rate of rewarming, o...

Ferrous and ferric sulphates and (oxy)hydroxides can grow on pyrite surfaces and slow oxidation rates. However, in northern climates, repeated freeze/thaw cycles can cause cracks, channeling and exposure of new surfaces. In the present study, weathering in a repeated freeze/thaw experiment was compared to a regular humidity cell experiment. Introductory results (after 17 weeks) show very small ...

The purpose of this study was to determine the negative effects (cryodamage) on human spermatozoa after freeze-thawing and to determine whether freeze-thawing of spermatozoa with a programmed slow freezer is better than freezing with liquid nitrogen vapour (rapid freezing) with regard to alterations in sperm chromatin and morphology in semen from fertile (donor) and subfertile, IVF/ICSI, patien...