retinoic acid (ra) plays a key role in pattern formation along the major body axis and limbformation during vertebrate development. exposure to excessive retinoic acid (in uterus) generatescongenital malformations in limbs, craniofacial, cns, urogenital, heart, and axial skeletons. in thesestudies, seven groups of nmri pregnant mice were administered a single gavage dose of 100 mg /kgbody weigh...

introduction: determining the skeletal age and remaining growth potential of patients are important factors in orthodontic treatment. evaluating cervical vertebrae development is a reliable method for determining skeletal age. this study aimed to evaluate the correlation between dental calcification and stages of skeletal maturation. materials and methods: this descriptive cross-sectional study...

The ossification centers onset of the quail vertebrae, ribs, and sternum in embryos and hatchling birds was studied. Specimens were cleared, stained with Alcian Blue and Alizarin Red S and examined using stereomicroscope. The chondral rudiments of the vertebrae were observed at the 6th day of incubation (E6). The osteogenesis of the vertebrae was accomplished with both perichondral a...

martin kirschner (1879-1942), versatile german surgeon who had his scientific research and academic interests addressed topics which are covered by several specialities such as general surgery, orthopaedic surgery, neurosurgery, anesthesiology and plastic surgery. he also was the editor of five medical journals and autjor of 249 articles and eight textbooks. his greatest and lasting contributio...

Introduction: Skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. The aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. Methods: In this pa...

abstract purpose: skeletal staining is a way to study the effect of many chemical or herbal substances on development of bones and cartilages in order to record the level of probable deformity.this article aimed to present a modified protocol to make all skeletal studies on laboratory animals possible. materials and methods: the important notes about skeletal staining were fixing by ethanol and...