Three techniques for estimating rumen protein degradability were used together with in vivo apparent nitrogen digestibility estimates from sheep fed dried chopped Pangola grass or Siratro. The techniques used were 1) nitrogen disappearance from nylon bags suspended in the rumen, 2) S labelling of rumen microbial protein with plant nitrogen degradability calculated as the difference between micr...

rhizospheres inoculated with strain PBK3-LS, 48% of the dibenzofuran was remoyed within 3 days (initia] substrate, 25 pg). Similar resutts were obtained in soilcultured siratro rhizospheres using sterite yermiculite. When non-sterile field seils were used instead of sterile vermiculite, the inocu]ated recombinant strain could grow on the siratro root in all soils tested, except for wet paddy fi...

Four ruminally cannulated wethers (31+/-1.3kg) were used in an experiment with a 4x4 Latin square design to estimate the DM intake, apparent digestibility, nitrogen balance, rumen ammonia and microbial protein production. The sheep had ad libitum access to either Cassia rotundifolia (Cassia), Lablab purpureus (Lablab), Macroptilium atropurpureum (Siratro) or Stylosanthes guianensis (Stylo). Dry...

The soluble, total-bound and ®bre-bound condensed tannins (CT) in Cassia rotundifolia (cassia), Lablab purpureus (lablab) and Macroptilium atropurpureum (siratro) grown in tropical and subtropical regions were determined by the butanol±HCl method. Cassia had the highest total CT content of 29.5 g/kg DM than that of lablab, 16.9 g/kg DM which was intermediate while that of siratro, 12.4 g/kg DM,...

Rhizobia classified as Bradyrhizobium spp. comprise a highly heterogeneous group of bacteria that exhibit differential symbiotic characteristics on hosts in the cowpea miscellany cross-inoculation group. To delineate the degree of specificity exhibited by four legumes in the cowpea miscellany, we tested the symbiotic characteristics of indigenous cowpea bradyrhizobia on cowpea (Vigna unguiculat...

agricultural sustainability is closely related with the efficient use of natural resources, which are primarily transformed by the action of microorganisms. soil microorganisms are usually used as early indicators of soil quality since they rapidly respond to changes in soil management. a field experiment was carried out aiming to evaluate the effect of siratro (macroptylium atropurpureum), bah...

Parasponia, a woody member of the elm family, is the only nonlegume genus whose members are known to form an effective nitrogen-fixing symbiosis with a Rhizobium species. The bacterial strain RP501 is a slow-growing strain of Rhizobium isolated from Parasponia nodules. Strain RP501 also nodulates the legumes siratro (Macroptilium atropurpureum) and cowpea (Vigna unguiculata). Using a cosmid clo...

Alternative oxidase activity (cyanide-insensitive respiration) was measured in mitochondria from the shoots, roots, and nodules of soybean (Glycine max L.) and siratro (Macroptilium atropurpureum) plants. Activity was highest in the shoots and lowest in the nodules. Alternative oxidase activity was associated with one (roots) or two (shoots) proteins between 30 and 35 kilodaltons that were dete...

Decreased solar radiation due to cloud cover or shading by plantation crops or associatedass can severely limit the production of tropical forage legumes. We therefore evaluated the response of six legumes (three replicated and three not replicated) to four radiation regimes (100, 70, 45, and 27% of unshaded solar radiation, hereafter termed "full sun") with polypropylene netting in the field. ...

We examined the genetic structure and symbiotic characteristics of Bradyrhizobium isolates recovered from four legume species (Lupinus albus [white lupine], Lupinus angustifolius [blue lupine], Ornithopus compressus [yellow serradella], and Macroptilium atropurpureum [sirato]) grown in an Oregon soil. We established that multilocus enzyme electrophoresis (MLEE) can provide insights into the gen...