nucleolar organizer regions (nor) are defined as nucleolar components containing a set of argyrophilic proteins which are selectively stained by colloidal silver nitrate staining. although studies have shown that the number of nor dots or particles is directly related to the rapidity of cell proliferation in cancer cells, prognostic or diagnostic value of nor remains controversial. the aim of t...

The mechanism by which silver staining of proteins in polyacrylamide gels interferes with mass spectrometry of peptides produced by proteolysis has been investigated. It was demonstrated that this interference increases with time between silver staining and gel processing, although the silver image is constant. This suggested an important role of the formaldehyde used in silver staining develop...

Paraffin sections were deparaffinized and hydrated to distilled water, then placed in 20% silver nitrate and kept in 60°C for 15 minutes, then rinsed in distilled water. Then sections were treated with formalin solution containing ammoniacal silver solution for about 15 min, followed by rinsing and placing tissue in sodium thiosulfate solution for 2 minutes and washing in tap water. The section...

10. Peters A. Experiments on the mechanism of silver staining. II. Development. Quart J Microsc Sci. 1955;96:103-115. 11. Kiernan JA. Silver staining of axons in subcellular fractions of nervous tissue. Experientia. 1970;26:1352. 12. Grizzle WE. Theory and practice of silver staining in histopathology. J Histotechnol. 1996;19(3):183-195. 13. Clark G. Staining Procedures used by the Biological S...

conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring th...

the indian riverine buffaloes are more susceptible to variations in the environment. these variations affect seminal plasma proteins, which in turn affect fertility. this study was conducted to evaluate variations in the indian bubalus bubalis seminal plasma protein profile using two-dimensional polyacrylamide gel electrophoresis (2d-page) during winter and summer seasons, and its relation with...

A rapid and easy method for staining lipopolysaccharides with ethidium bromide is described. Lipopolysaccharides could be visualized by ethidium bromide with almost the same sensitivity as found with the silver-staining method in less than 30 min. The ethidium bromide-staining method was particularly suitable for staining lipopolysaccharides possessing acidic O-specific polysaccharides, which w...