BACKGROUND The present study is aimed to assess the usefulness of silver nitrate staining of nucleolar organizer regions (NORs) as a quantitative criterion for the diagnosis of selected head and neck tumors. MATERIALS AND METHODS The silver nitrate staining technique was used on 195 paraffin blocks collected from 85 patients. The samples consisted of 21 squamous cell carcinoma (SCC) of larynx...

Silver nitrate staining of decalcified bone sections is known to reveal osteocyte canaliculi and cement lines. Nucleolar Organising Regions (NOR) are part of the nucleolus, containing argyrophilic proteins (nucleoclin/C23, nucleophosmin/B23) that can be identified by silver staining at low pH. The aim of this study was to clarify the mechanism explaining why AgNOR staining also reveals osteocyt...

background and aims. several diagnostic methods are being employed to detect benign and malignant lesions, one of which is silver nitrate staining for organizer regions. the number of nucleolar organizing regions (nors) can be used to show the degree of cell activity or metabolism in pathologic lesions. this study was designed to evaluate nors as determinants of precancerous and squamous cell c...

Silver staining was introduced more than a decade ago as a sensitive procedure to detect trace amounts of proteins in polyacrylamide gels (1). Since then, silver staining has been perfected and extended to the study of other biological molecules that have been separated by electrophoresis in a variety of supports (2,3). Silver staining follows generally one of two methods. One uses diamine or a...

Background: Oral spirochetes play a definitive role in the etiology of periodontitis. But their study is hampered due to difficulty in cultivating them and molecular methods are expensive. Hence the study explores the possibility of using staining as a screening procedure for the study of oral spirochetes. Experimental approach: The study included 100 patients with chronic periodontitis and 100...

Sections from the human somatosensory cortex were observed with a light microscope. The neurons were classified into light and dark ones. The light neurons were slightly stained with thionin, luxol fast blue MBS and azocarmine G (80% of all neurons). The dark neurons were more or less shrunken, and stained intensely with these dyes (20% of all neurons). Double staining with luxol fast blue MBS ...

Isolated blastomeres from 8- to 16-cell-stage embryos were fused by standard micromanipulatory means with either unfertilized eggs or fertilized or haploid parthenogenetically activated pronuclear-stage embryos. The hybrid eggs/embryos were incubated overnight in the presence of Colcemid until they had entered the first cleavage division. Air-dried chromosome preparations were then stained with...

BACKGROUND AND AIMS Several diagnostic methods are being employed to detect benign and malignant lesions, one of which is silver nitrate staining for organizer regions. The number of nucleolar organizing regions (NORs) can be used to show the degree of cell activity or metabolism in pathologic lesions. This study was designed to evaluate NORs as determi-nants of precancerous and squamous cell c...

There is an increasing interest in using of silver coated (silver nitrate, silver alloy, silver oxide) catheters for hospital patients. Clinical trials with silver-coated urinary catheters have shown conflicting results. The most often performed catheterization is for a short period of time. The above observations have encouraged the authors to investigate the influence of silver nitrate (AgNO3...