This study explored food allergy caused by eating silkworm (Bombyx mori L.) pupae, a traditionally accepted food and animal feed in East and Southeast Asia, and identified two new allergens by proteomic and immunological methods. Proteins isolated from silkworm pupae were separated by two-dimensional gel electrophoresis (2-DE); pooled sera from patients allergic to silkworm pupa proteins were u...

Boiled silkworm pupa is a traditional food in Asia, and patients with silkworm pupa food allergy are common in these regions. Still now only one allergen from silkworm, arginine kinase, has been identified. The purpose of this study was to identify novel food allergens in silkworm pupa by analyzing a protein extract after heat treatment. Heat treated extracts were examined by proteomic analysis...

BACKGROUND The silkworm, Bombyx mori, is an important insect in the textile industry and its pupa are used in Chinese cuisine and traditional Chinese medicine. The silk, urine and dander of silkworms is often the cause of allergies in sericulture workers and the pupa has been found to be a food allergen in China. Recent studies have focused on reporting cases of silkworm allergies, but only a f...

Human 29IJ6 IgG was expressed in silkworm using a Bombyx mori 25 nucleopolyhedrovirus (BmNPV) bacmid system. The mean amounts of 296IJ6 IgG 26 produced in larval hemolymph and whole pupae were 30.1 μg/larva and 78.0 μg/pupa,

Waste silkworm pupae (SWP) generate vast resources of nutrients for livestock and poultry. In the present investigation, three days old chicks of RIR strain were allocated to five dietary treatments of silk worm pupae meal. The energy budget was prepared from calculated proximate analysis and growth performance of broiler chicks fed with different percentages of silk worm pupae. The result show...

Recently, use of naturally-occurring low-cost and harmless material for removal of trace contaminants from colored wastewater has attracted extensive attention. In this study, silkworm pupa has been used as a basic dye adsorbent. Adsorption capacity and isotherms have been assessed. Results show that adsorption isotherms can be expressed well by Langmuir equation. The effects of parameters such...

A novel sodium N-fatty acyl amino acid (SFAAA) surfactant was synthesized using pupa oil and pupa protein hydrolysates (PPH) from a waste product of the silk industry. The aliphatic acids from pupa oil were modified into N-fatty acyl chlorides by thionyl chloride (SOCl2). SFAAA was synthesized using acyl chlorides and PPH. GC-MS analysis showed fatty acids from pupa oil consist mainly of unsatu...

Human growth hormone (hGH) is a peptide hormone secreted by eosinophils of the human anterior pituitary, and a regulatory factor for a variety of metabolic pathways. A 30-kD protein from the pupa stage of silkworm was detected by Western blotting and confirmed by immunoprecipitation based on its ability to bind to anti-hGH antibody. This protein, named BmhGH-like protein, was purified from fres...

This article proposes the influence of cocoon drying technology on properties shell and pupa silkworm. We also analyzed change in initial weight cocoons treated at different temperatures, microscopic view shells. Cocoons grown times year were taken as object study. As a result treatment with an infrared ray, hot air chemical methods, yield, color solubility compared analyzed.

The silk of silkworm consists of fibroin fiber coated by sericins. In addition, some nonprotein components were also identified in the sericin fraction. The presence of nonprotein components in the silk has not been well explained. In the present study, methods based on gas chromatography-mass spectrometry were used to identify the metabolites in the cocoon silk from a wild silkworm and two dom...