optimization of the condition for pcr-directed sequencing of microsatellites poly adenine (a) length polymorphisms is more difficult and sensitive compared with other common sequences. replication slippage may occur for polymerase enzyme during microsatellite amplification and direct sequencing of these pcr products will be challenging for heterozygote samples. so, the aim of this study is to i...

dna typing is a new method with important applications in forensic medicine. in the present study, we evaluated application of dna typing in iran. loci hum lpl, hum tpox, hum f13, hum vw 31a, hum th01 and hum fes/fps of dna short tandem repeats were studied. to determine sensitivity of the test, 85 mother-child couples (1020 chromosomes) that were referred to dna section of legal medicine organ...

Short-sequence DNA repeat (SSR) loci can be identified in all eukaryotic and many prokaryotic genomes. These loci harbor short or long stretches of repeated nucleotide sequence motifs. DNA sequence motifs in a single locus can be identical and/or heterogeneous. SSRs are encountered in many different branches of the prokaryote kingdom. They are found in genes encoding products as diverse as micr...

a reliable dna extraction method for use on extra virgin olive oil based on a commercial kit was defined, and the possibility of using this dna for fingerprinting the original cultivar was demonstrated. the genetic traceability of single-cultivar virgin olive oil from two cultivars (carolea and frantoio) was achieved by identifying the varieties from which they were produced. this involved the ...

out of fifty-two iranian nonaflatoxigenic strains of aspergillus flavus, which were collected from various substrates (soil and kernel) and sources (peanut, corn and pistachio), fifteen representatives were selected according to their different geographical origins (six provinces: guilan and golestan, ardebil, fars, kerman and semnan) and vegetative compatibility groups (vcgs, ir1 to ir15) for ...