punicagranatum cv. malassaveh is one of the most desirable commercial cultivars in iran. in thisstudy some experiments were conducted to introduce the best hormonal treatments for directadventitious shoot regeneration from mentioned cultivar via cotyledonary explants. for thispurpose, both liquid and agarmurashige and skoog (1962) (ms) medium supplemented with baat 2, 4.5, 9, 13 μm alone and in...

Regeneration, a remarkable example of developmental plasticity displayed by both plants and animals, involves successive developmental events driven in response to environmental cues. Despite decades of study on the ability of the plant tissues to regenerate a complete fertile shoot system after inductive cues, the mechanisms by which cells acquire pluripotency and subsequently regenerate compl...

conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. a general prerequisite for these approaches is to establish an efficient plant reg...

In order to select the best tomato cultivar for Agrobacterium mediated gene transformation studies, In vitro regeneration frequency of hypocotyls, leaf disc and shoot tip of five tomato cultivars (Lycopersicon esculentum) was investigated on a regeneration medium supplemented with 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. Significant differences in regeneration capacity between genotypes...

This experiment was performed to optimize the medium for tissue culture of Taraxacum kok-saghyz Rodin. Different tissue culture approaches such as shoot regeneration from seed, callus formation from leaf explants and plant regeneration from callus were investigated in this study. All the explants were cultured on MS basal medium supplemented with 20g/l sucrose, 7g/l agar and different plant gro...

Conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. Genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. A general prerequisite for these approaches is to establish an efficient plant reg...

Functional screening of an Arabidopsis cDNA library enabled the identification of a novel cDNA, ESR1 (for Enhancer of Shoot Regeneration), that can confer cytokinin-independent shoot formation when overexpressed in Arabidopsis root explants. Neither callus induction nor root formation was affected by ESR1 overexpression. ESR1 encodes a putative transcription factor with an AP2/EREBP domain. Sur...

Tissue culture provides a useful system to investigate how plant hormones are involved in this process. Auxin and cytokinin are widely used in plant regeneration. Gibberellin is also an important plant hormone in regulating plant growth and development. It is interesting to know the effects of gibberellin and its signalling pathway on plant regeneration. In this report Arabidopsis thaliana land...

Agrobacterium-mediated transformation of indica rice has been established in only a limited number of cultivars because the regeneration of plants from transformed embryogenic calli is highly cultivar-specific. Establishment of a highly efficient plant regeneration system from shoot apex explants applicable to many cultivars of indica rice will accelerate the application of transformation techn...

De novo shoot regeneration is widely used in fundamental studies and agricultural applications. Actin microfilaments are involved in many aspects of plant cell division, cell morphogenesis and cell signal transduction. However, the function of actin microfilaments during de novo shoot regeneration is poorly understood. Here, we investigated the organization of actin microfilaments during this p...