cholera toxin b subunit (ctxb) is a homopantameric, nontoxic subunit of cholera toxin that is responsible for its binding to the cell and has been known as a mucosal adjuvant for vaccines that could increase homoral and mocusal immunity response. in this work, the ctxb gene was fused to the stxb gene from shigella dysenteriae type i a vaccine antigen candidate against this pathogen, by a nonfur...

background: essential oils and their major constituents are useful sources of antimicrobial compounds. there are a few reports on the decontamination and antimicrobial activity of essential oils towards shigella spp. m aterials and methods: in this study, the antimicrobial and decontamination potentials of essential oils at different concentrations, belonging to plants such as thymus vulgaris, ...

Introduction: Awareness of the species of Shigella has particular importance in the way of dealing with an outbreak, controlling it, and treating patients. The purpose of this study was to use the Multiplex PCR method and comparison with the culture method to detect Shigella species, as well as analysing the antibiotic resistance pattern in its different species. Methods: Simultaneously, the fe...

shigellosis is one of the important gastrointestinal bacterial infections, particularly among children of developing countries such as iran. antibiotic susceptibility pattern and genetic typing for epidemiological purposes are of significant issues in shigella infectious control. the aim of this study was to investigate the antibiotic susceptibility and genetic relationship among shigella strai...

Shigella species and Escherichia coli are closely related organisms. Early phenotyping experiments and several recent molecular studies put Shigella within the species E. coli. However, the whole-genome-based, alignment-free and parameter-free CVTree approach shows convincingly that four established Shigella species, Shigella boydii, Shigella sonnei, Shigella felxneri and Shigella dysenteriae, ...

OBJECTIVES Shigellosis is an important cause of bloody diarrhoea in all age groups, especially in children. A retrospective study was done to analyse the pattern of shigella isolates and the antimicrobial susceptibility trend of these shigella isolated at different hospitals of Nepal from Jan, 2003- Dec, 2005. MATERIALS AND METHODS A total of 118 Shigella species isolated at nine different ho...

objective: shigellosis is one of the most common causes of morbidity and mortality in children with diarrhea in developing countries. it is essential to assess the antibiotic resistance patterns of these bacteria. ipah gene is one of the virulence factors which can be used for detection of shigella spp. materials and methods: total of 100 isolates of shigella were collected from different prov...

Shigellosis produces inflammatory reactions and ulceration on the intestinal epithelium followed by bloody or mucoid diarrhea. It is caused by enteroinvasive E. coli (EIEC) as well as any species of the genus Shigella, namely, S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. This current species designation of Shigella does not specify genetic similarity. Shigella spp. could be easily dif...

BACKGROUND Shigella species are among the common causes of bacterial diarrhoeal diseases. Traditional detection methods are time-consuming resulting in delay in treatment and control of Shigella infections thus there is a need to develop molecular methods for rapid and simultaneous detection of Shigella spp. In this study a rapid multiplex PCR were developed for simultaneous detection of three ...

The incidence of Shigella spp. was assessed in 877 infants from the public hospital in Rondônia (Western Amazon region, Brazil) where Shigella represents the fourth cause of diarrhea. Twenty-five isolates were identified: 18 were Shigella flexneri, three Shigella sonnei, three Shigella boydii and one Shigella dysenteriae. With the exception of S. dysenteriae, all Shigella spp. isolated from chi...