PURPOSE To develop a reproducible method for expanding mouse corneal-limbal epithelial cells and determine the role of extracellular Ca(2+) concentration and serum in modulating their growth and differentiation. METHODS Intact and viable corneal epithelial sheets were isolated from CD-1 albino mouse eyeballs by incubating for 18 hours at 4 degrees C in 15 mg/mL dispase II with sorbitol in def...

PURPOSE To compare the effectiveness of three culture media for growth, proliferation, differentiation, and viability of ex vivo cultured limbal epithelial progenitor cells. METHODS Limbal epithelial progenitor cell cultures were established from ten human corneal rims and grew on plastic wells in three culture media: supplemental hormonal epithelial medium (SHEM), keratinocyte serum-free med...

PURPOSE Transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on epithelially denuded amniotic membrane (dAM) in supplemented hormonal epithelial medium (SHEM) is an alternative solution for treating corneal blindness due to limbal stem cell (SC) deficiency. Because the phenotype of limbal niche cells (NCs) is preserved better in serum-free modified embryonic stem cell ...

In this paper, an improved expectation maximization (EM) algorithm called statistical histogram based expectation maximization (SHEM) algorithm is presented. The algorithm is put forward to overcome the drawback of standard EM algorithm, which is extremely computationally expensive for calculating the maximum likelihood (ML) parameters in the statistical segmentation. Combining the SHEM algorit...

This paper combines quasi-Z-source into a typical five-level inverter, which includes two dc voltage sources, two quasi-Z-sources and five switching devices. In this structure, the output voltage amplitude is not limited to dc voltage source and it can be increased by quasi-Z-source. Besides, due to nature of Z-source families, this new structure is reliable and higher efficiency. Also, in this...

PURPOSE To explore new strategies for effective isolation, preservation, and expansion of human corneal endothelial cells (HCECs). METHODS Human corneal Descemet's membrane and corneal endothelial cells were digested with collagenase A or Dispase II in supplemented hormonal epithelial medium (SHEM) for 1.5 to 16 hours. HCEC aggregates derived from collagenase A digestion were preserved in ser...

Author(s): Cozzi, Nicholas; Schiebout, Jessen; Leckrone, Dave; Marks, Amy; Goldstein, Corey; Purim-Shem-Tov, Yanina; Dugal, Brian; Bodnar, Sophia; Martin, Jerome; Sompalli, Vinootna; Lafleur, Crystal; Plattner, Haley; Murica, Hans; Mati, William; Hondros, Louis; Ward, Edward

Programme de l’année 2019-2020 : I. Joseph ben Shem Tov (Espagne, XVe s.) et la tradition philosophique l’union avec l’Intellect agent. — II. Juda Rome (Italie, XIVe s.), Commentaire du Qaddiš Qedušah.

Commentary on: Shem Patyna, A. Douglas Laird, Dirk B. Mendel, Anne-Marie O'Farrell, Chris Liang, Huiping Guan, Tomas Vojkovsky, StefanVasile,XueyanWang,JeffreyChen,MarenGrazzini,Cheng Y. Yang, Joshua € O. Haznedar, Juthamas Sukbuntherng, Wei-Zhu Zhong, Julie M. Cherrington, and Dana Hu-Lowe. SU14813: a novel multiple receptor tyrosine kinase inhibitor with potent antiangiogenic and antitumor ac...