background: the antifertility effect of ampicillin (amp, 40 mg/kg) and sulphasalazine or salicylazosulfapyridine (sasp, 300,450 and 600 mg/kg) in male rats has been reported earlier. the combination of amp and sasp is generally used in certain pathological conditions, but the combined effect of these two drugs on the fertility is not clear. so, the aim of this study was to investigate the antif...

BACKGROUND Analog-based free testosterone test results, sex hormone binding globulin (SHBG) concentrations, and total testosterone concentrations are somehow related. This study used new experiments to clarify these relationships. METHODS An analog-based free testosterone immunoassay and a total testosterone immunoassay were applied to well-defined fractions of serum testosterone. First, they...

an enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (t-3-0-cmo-bsa ) and penicillinase-labelled t-3-0-cmo was developed. this assay was utilized to measure testosterone in serum samples of male and female subjects. the sensitivity of the assay is 50pg/well and the antibody developed crossreacted...

An enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (T-3-0-CMO-BSA ) and penicillinase-labelled T-3-0-CMO was developed. This assay was utilized to measure testosterone in serum samples of male and female subjects. The sensitivity of the assay is 50pg/well and the antibody developed crossrea...

A young female with prolonged intermenstrual bleeding was found to have raised total plasma testosterone of 25.8 nmol/l (NR<2.9 nmol/l) using the Roche Elecsys Testosterone I immunoassay without clinical features of virulisation. Few months ago investigations for lethargy and low libido had shown normal total testosterone of 0.8 nmol/l. Further history revealed that she was using maca extract t...

Investigating the interference of danazol in testosterone radioimmunoassays, we measured serum testosterone concentrations in the serum of women receiving danazol, with three testosterone radioimmunoassay kits. All of these showed positive interference by danazol or its metabolites (or both). Values for testosterone obtained with two of the methods (Diagnostic Products Corp. and Farmos Diagnost...

We describe a problem in measuring serum testosterone concentrations in the serum of women who are taking danazol. The Diagnostic Products Corporation testosterone radioimmunoassay gives very high apparent testosterone concentrations in these women and the value obtained depends on the volume of serum extracted per assay tube. Chromatography of the serum extracts on Sephadex LH-20, but not on C...

17 α Methyl testosterone is a synthetic steroid hormone having androgenic properties. It used in aquaculture, medical treatments, sports as doping drugs, improving food efficacy but its residue meat and their products can cause risk to the human, animals environment. In this article, we have focused on developing homologous ELISA using 17α testosterone-3-Carboxymethyloxime-Bovine serum albumin ...

In this method, we use the Amicon MPS-1 centrifugal ultrafiltration device and the YMB membrane in measuring free testosterone in serum. Two independent assays are combined: total testosterone and the ultrafiltrable fraction of added [3H]testosterone. The unbound fraction is determined in 0.15-0.5 mL ultrafiltrates of 0.6 to 1 mL of variably diluted serum that has been equilibrated with [3H]tes...