aim : the purpose of this study was to characterize phenotypically and genotypically the serotypes of the e. coli o111 associated with diarrheal disease and assess the variation among serotypes in terms of specific virulence factors and hela cells adherence patterns. background : escherichia coli o111 serogroups are prevalent in endemic or sporadic cases of diarrhea, especially in developing ar...

Shiga toxin-producing Escherichia coli (STEC) O111:NM is an important serotype that has been incriminated in disease outbreaks in the United States. This study characterized cattle STEC O111:NM for virulence factors and markers by PCR. Major conclusions are that STEC O111:NM characterized in this study lacks stx2 and the full spectrum of nle gene markers, and it has an incomplete OI-122.

A microagglutination (MA) assay to identify antibodies to Escherichia coli O111 and O157 was conducted in sera collected from 60 patients during a food-poisoning outbreak affecting 181 patients in Japan which was caused by the consumption of contaminated raw beef. Enterohemorrhagic E. coli (EHEC) O111:H8 and/or O157:H7 was isolated from the stools of some of the patients, but the total rate of ...

A verotoxin-producing Escherichia coli serotype O111:NM strain (strain 10049; verotoxin 1 positive) persistently infected experimentally inoculated gnotobiotic pigs, causing attaching-effecting intestinal lesions and chronic diarrhea. Experiments were performed to determine whether persistent infection might be associated with immunocompromise of the host of this organism. Pigs inoculated with ...

Background: E. coli is one of the most important zoonotic pathogen and also an indicator faecal contamination food water. There paucity data regarding level raw meat characterization pathogenic from slaughtered cattle chicken in un-organized sector Mizoram, India. Methods: Raw samples traditionally were collected Aizawl, Kolasib Champhai districts Mizoram analyzed for count (ECC), serotypes vir...

background and objectives: dairy cattle have been implicated as principal reservoir of verotoxin-producing escherichia coli (vtec), with undercooked ground beef and raw milk being the major vehicles of food borne outbreaks. vtec has been implicated as an etiological agent of individual cases and outbreaks in developed countries. this study was designed to determine the prevalence of vetec in di...

AIM The purpose of this study was to characterize phenotypically and genotypically the serotypes of the E. coli O111 associated with diarrheal disease and assess the variation among serotypes in terms of specific virulence factors and HeLa cells adherence patterns. BACKGROUND Escherichia coli O111 serogroups are prevalent in endemic or sporadic cases of diarrhea, especially in developing area...

Shiga toxin–producing Escherichia coli (STEC) strains are important foodborne pathogens. Among these, E. coli O157:H7 is the most frequently isolated STEC serotype responsible for foodborne diseases. However, the non-O157 serotypes have been associated with serious outbreaks and sporadic diseases as well. It has been shown that various STEC serotypes are capable of forming biofilms on different...

Serotyping of Shiga toxin-producing Escherichia coli (STEC) has been contingent upon the availability of antisera. Here we describe a 7-plex microbead-based immunoassay to simultaneously serotype seven STECs (i.e., belonging to serogroups O26, O45, O103, O111, O121, O145, and O157) by the Luminex xMAP® technology. This technology presents many advantages: Its multiplexed format (up to 100 analy...

The cell surfaces of five enteropathogenic Escherichia coli serotypes (O111:H2; O111:H12; O125:H9; O119:H6; O26:H11) were assayed by chemical methods, lectin agglutination tests and spectroscopy associated to transmission electron microscopy. Results of lectin agglutination assays showed that all strains reacted with mannosebinding lectins. Strains belonging to serotype O125:H9 also agglutinate...