This study was designed to compare the effect of straw size (0.25 vs. 0.5 ml) and thawing time (30 vs. 60 sec) on the quality of cryopreserved buffalo bull semen. Sperm motility, plasma membrane integrity and viability were higher (p ≤ 0.05) in 0.25 ml than 0.5 ml straw, thawed at 37°C either for 30 or 60 sec. In conclusion, cryopreservation of buffalo semen in 0.25 ml straw resulted in a highe...

A series of experiments were conducted to determine the suitable freezing and thawing temperatures for the freezing of boar semen in 5 ml maxi-straws. The ultrastructure, in vitro fertilization (IVF) and artificial insemination (AI) of frozen-thawed semen were also be evaluated. The 5 cm freezing height gave the best results not only in post-thaw motility rate (54.00%), but also in normal acros...

The objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches. The ejaculates were split-sampled and diluted at 37°C with tris-citric acid extender having 15x...

a 6-year-old lactating holstein cow with a history of repeat breeding syndrome (failed to conceive afterthree artificial inseminations (ai) with fertile semen) was referred to the veterinary teaching hospital of theschool of veterinary medicine, shiraz university. according to the history, neither abnormal clinical signs,nor uterine discharge had been observed subsequent to ai. the cow had show...

An improvement in avian semen cryopreservation is essential and has the potential to improve the cryo-gene banking efficiency. This study compared two cryopreservation methods (slow freezing and vitrification) and the effect of different thawing/warming temperatures (5 ̊C, 25 ̊C and 41 ̊C) on Venda cockerel’s spermatozoa. Semen samples from Venda cockerels were diluted with modified Kobidil extend...

Cryopreservation of few spermatozoa is still a major challenge for male fertility preservation. This study reports use a new micro-straw (LSL straw) for freezing few spermatozoa for intracytoplasmic sperm injection (ICSI). Semen samples from 22 fertile donors were collected, and each semen sample was diluted and mixed with cryoprotectant in a ratio of 1:1, and then frozen using three different ...

The objective was to determine the effect of sequence of insemination after simultaneous thawing of multiple 0.5 mL semen straws on conception rate in suckled multiparous Nelore cows. The effect of this thawing procedure on in vitro sperm characteristics was also evaluated. All cows (N = 944) received the same timed AI protocol. Ten straws (0.5 mL) of frozen semen from the same batch were simul...

The objective of this study was to compare fixed-time AI pregnancy rate in Angus crossbred beef cows inseminated with frozen-thawed or fresh-extended semen. Two ejaculates from each of two Angus bulls were collected by artificial vagina and pooled for each bull. The pooled semen from each bull was divided into two aliquots; Aliquot 1 was extended using Caprogen (LIC, Hamilton, New Zealand) to a...

Nine ejaculates were extended, placed in .5ml French straws and frozen vertically in two automatic forced vapor freezers (Linde BF-4 and FC-3) at three loads (2,500, 5,000 and full capacity of 7,500 straws in 5-straw goblets on canes). For each load, six locations within each freezing chamber were compared: upper and lower levels of the center, intermediate and corner areas. Semen was extended ...

OBJECTIVE The present study was designed to determine the effect of the freeze-thawing procedure, computer controlled rate freezing and duration for six months, on human sperm chromatin (assessed by acridine orange test), vitality, motility, and morphology. DESIGN Experimental study MATERIAL AND METHOD Twenty semen samples were obtained from patients attending the infertility unit. The seme...