Single radial immunodiffusion (SRID) assay requires a reference antigen and an antibody to the hemagglutinin (HA) of an influenza vaccine. As it takes 2‑3 months to develop the reference antigen, vaccine development is delayed in cases of an influenza pandemic. In the present study, the measurement of the HA content of influenza vaccines was assessed using size exclusion high performance liquid...

Selenium (Se) is an essential element for animals, including fish. Due to changes in feed composition for Atlantic salmon (Salmo salar), it may be necessary to supplement feeds with Se. In the present work, the transfer of Se and Se species from feed to muscle of Atlantic salmon fed Se supplemented diets was studied. Salmon were fed basal fish feed (0.35 mg Se/kg and 0.89 mg Se/kg feed), or fee...

Batch leaching tests were employed to investigate the leaching potential of arsenic (As) and selenium (Se) from two fly ash samples. The effect of pH and solid-to-liquid (S/L) ratio on As and Se leaching was examined in this preliminary study. Maximum As leaching occurred at pH 7-8, and soluble As concentration slightly increased with decrease of S/L ratio. Se displayed consistently increasing ...

BACKGROUND It had been demonstrated that sugars from various plants can act as potent agents, which induce apoptosis of cancer cells. METHODS Using HPLC, we fractionated a mixture of two plant extracts from the plant family Solanaceae, namely Capsicum chinense and the plant family Amaryllidaceae namely Allium sativum. We evaluated the effect of different fractions on apoptosis of HepG2 cell l...

Thiol-containing compounds such as glutathione, cysteine and homocysteine react with selenite under specific conditions to form selenotrisulfides of significance in cellular systems. This study describes the synthesis and mass spectrometric characterization of selenotrisulfides of glutathione (GSH), cysteine (Cys) and homocysteine (HCys) by electrospray-MS (ESI-MS). The synthesized species are ...

Human erythrocyte transketolase (sedoheptulose-7-phosphate: D-glyceraldehyde-3-phosphate, glycolaldehyde transferase, E.C. 2.2.1.1.) has been isolated from erythrocytes with a specific activity of 59.84 U/mg. SDS-PAGE and SE-HPLC were used both as a measure of purity and as a preparative mean to obtain a higher degree of purity. Four protein bands corresponding to molecular weights of 32,0...

human erythrocyte transketolase (sedoheptulose-7-phosphate: d-glyceraldehyde-3-phosphate, glycolaldehyde transferase, e.c. 2.2.1.1.) has been isolated from erythrocytes with a specific activity of 59.84 u/mg. sds-page and se-hplc were used both as a measure of purity and as a preparative mean to obtain a higher degree of purity. four protein bands corresponding to molecular weights of 32,000, 3...

In recent years, an increasing number of reports have been focused on the structure and biological role of non-canonical nucleic acid secondary structures. Many of these studies involve the use of oligonucleotides that can often adopt a variety of structures depending on the experimental conditions, and hence change the outcome of an assay. The knowledge of the structure(s) formed by oligonucle...

Direct examination † Culture Molecular identification Ring of epithelial cells Giant cells Caseous necrosis Altered leukocytes Reference M. tuberculosis ± + IS6110 + + + – (6) M. avium 183 NR NR NR + NR + + NR NR (7) M. avium subsp. hominissuis 34 NR NR NR + IS1245 NR NR NR NR (5) M. haemophilum 39 NR NR + + 16S–23S rDNA + + + – (8) M. lentiflavum 3 SE Cured + + 16S rRNA + + NR + (9) M. bohemic...

Two modified methods for assaying sodium diclofenac were developed by GC and HPLC. Diclofenac was converted into methyl ester derivative by methyl iodide in acetone. The ester was extract and subjected to GLC with flame ionization detector. %5 SE-30/chrom W-HP (80-100 mesh) was used as a column in GC. For reversed phase HPLC, the mobile phase was methanol and water (55-45). The separation was p...