Siderophore-promoted iron acquisition by microorganisms usually occurs in the presence of other organic molecules, including biosurfactants. We have investigated the influence of the anionic surfactant sodium dodecyl sulfate (SDS) on the adsorption of the siderophores DFOB (cationic) and DFOD (neutral) and the ligand EDTA (anionic) onto goethite (alpha-FeOOH) at pH 6. We also studied the adsorp...

Glucanase enzyme isolated from Trichoderma isolates were purified to homogeneity using ammonium persulphate precipitation and Fast Protein Liquid Chromatography. Purity of the isolated enzyme was confirmed by SDS-PAGE. Enzymatic properties such as effect of temperature, pH, SDS and EDTA were determined. Molecular weight of Trichoderma isolates was found to be around 55 kDa. The optimum temperat...

thermophilic bacillus sp. gus1, isolated from  a soil  sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and zymogram analysis. the enzymes were stable in the alkaline ph range (8.0-12.0), with the optimum temperature and ph range of the proteases being 70ºc and 6.0-12.0, respectively. all th...

this study investigated the feasibility of using different extracting solutions at various concentrations to remove copper from low permeability silty loam soils, which have a high buffering soil, ph of 7.6, where it spiked with copper at concentration of about 1500 mg/kg to represent soil conditions. the most efficient extracting solutions could be used as purging/extracting solutions in other...

Thiol-disulfide isomerization in thrombospondin may affect the function of this adhesive protein. Two assays were developed to analyze the determinants of thiol-disulfide exchange and to correlate this exchange with thrombospondin conformation. (1) A competitive immunoassay for the EDTA-conformation of thrombospondin was developed with monoclonal antibody D4.6. (2) The free thiol(s) in thrombos...

Antigen Retrieval Overview  Overview of Heat-induced Epitope Retrieval Techniques and Devices Room Temperature Epitope Retrieval (RTER)  Hydrochloric Acid Method (pH 1)  Formic Acid Method (pH 2) Heat Induced Epitope Retrieval (HIER)  Citrate Buffer Method (pH 6)  Citrate-EDTA Buffer Method (pH 6.2)  EDTA Method (pH 8)  Tris-EDTA Method (pH 9)  TBS Method (pH 9)  Tris Buffer Method (pH...