background: typhoid fever is still one of the serious public health problems in many geographic areas and is endemic in most countries. aim of current study was to evaluate a shortened time –multiplex pcr for rapid detection of different sal monella enterica serovars. methods: the pcr primers for three target genes tyv, prt and inva were subjected for amplification by pcr. by using sim ple dna ...

conclusions these results show that retail meat and vegetables can serve as a reservoir of multiple antimicrobial resistant salmonella and can probably be a potential route of transmission of these pathogens into human population. results the prevalence of salmonella in retail pork, chicken meat, and fresh vegetables were 82% (34/41), 67.5% (27/40), and 46% (37/80), respectively. the salmonella...

salmonella enterica serotypes are one of the most important food borne pathogens and significant public health concerns around the world in humans and other animal species. a total of eighty three epidemiologically unrelated clinical isolates of salmonella enterica serovars were subjected to antimicrobial susceptibility testing. eleven isolates (13.1%) which were resistant to at least 4 groups ...

salmonella is a gram-negative, non-spore forming facultative anaerobic, motile bacilli. in developing countries, non-typhoidal salmonella (nts) accounts for the steadily increase in the proportion of human infections in recent decades. the clinical presentations of non-typhoidal salmonella infection are protean, including gastroenteritis (the most common one), bacteremia, septic arthritis, oste...

To facilitate the detection of Salmonella and to be able to rapidly and conveniently determine the species/subspecies present, we developed and tested a generic and differential FRET-PCR targeting their tetrathionate reductase response regulator gene. The differential pan-Salmonella FRET-PCR we developed successfully detected seven plasmids that contained partial sequences of S. bongori and the...

Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated...

Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically present in Salmonella were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and Salmonella bongori (V), and were clustered into 17 groups by their comparison pa...