to evaluate the effect of probiotic (primalac) and salmonella enteritidis-specific igy on prevention of salmonella enteritidis infection in broiler chickens, four 33 week-old single comb white leghorn hens were hyperimmunized with salmonella enteritidis (se) whole cell antigens obtained by ultrasonication and administrated at a protein concentration of 500 ?g/ml after centrifugation. primary im...

objective: the purpose of the study was to evaluate the production of antibodies in serum as well as egg yolk raised against s. typhimurium and the cross-reactivity of this antibody with other enteric bacteria. materials and methods: white egg–laying hens were immunized with s. typhimurium, heat-killed whole cell, in freund's adjuvant. immunization was done with 107 conlony forming unit (cfu) o...

background: during the last twenty years, the extraction of specific egg yolk (igy) antibodies from the immunized chickens has been accepted as a useful alternative to the immunization of mammals. the aim of the present study was immunizing the chickens with human umbilical cord serum (hucs) and the extraction of specific anti-human globulins (igg, c3b, and c3d) antibodies from egg yolk in orde...

introduction chicken is the only avian species in which polyclonal antibodies, like igg is transported from the hen to the egg yolk in a similar manner as the transport of mammalian igg from the mother to the fetus. immunoglobulin y in the chicken is transported to the egg and accumulates in the egg yolk in large quantities. igy is an egg yolk antibody that has been used widely for treatment an...

Salmonella enteritidis (SE) colonizes the intestinal tract of poultry and causes food born illness in humans. Reduction of (SE) colonization in the intestinal tract of poultry reduces potential carcass contamination during slaughter. The purpose of this study was to investigate the effect of SE-specific yolk immunoglobulin (IgY) on prevention of SE colonization in orally infected broiler chicke...

AIM This study was undertaken to compare the immunoglobulin Y (IgY) level and its efficacy in laying hens of four different breeds of poultry (viz., Vanraja, Gramapriya, BlackRock, and KalingaBrown) and its relative transfer in egg yolk and chick. MATERIALS AND METHODS This study was conducted in 48 apparently healthy laying hens vaccinated with Salmonella inactivated polyvalent vaccine, eggs...

The present study was an attempt to raise hen egg yolk Ig (IgY) simultaneously directed against Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) in the same egg yolk. The immunopotentiating effect of 2 different adjuvants -- Freund's adjuvants (FA) and immunostimulating complexes matrix (IM) -- on antibody response was also evaluated. Bacterial outer membrane proteins (OMP) were sele...

Avian embryos and neonates acquire passive immunity by transferring maternal immunoglobulins from serum to egg yolk. Despite being a convenient source of antibodies, egg yolk immunoglobulins (IgY) from immunized hens have so far received scant attention in research. Here we report the generation and rapid isolation of IgY from the egg yolk of hens immunized against the alpha subunit of the huma...