BACKGROUND Spinosad is a macrolide antibiotic produced by Saccharopolyspora spinosa with aerobic fermentation. However, the wild strain has a low productivity. In this article, a computational guided engineering approach was adopted in order to improve the yield of spinosad in S. spinosa. RESULTS Firstly, a genome-scale metabolic network reconstruction (GSMR) for S.spinosa based on its genome...

Creatine modification for commercialization has included many variations to increase its stability. The current report assesses the stability of a sodium bicarbonate buffered creatine sold under the brand name Kre-Alkalyn under various conditions. Kre-Alkalyn is shown to have a good 6 year stability and shelf life under accelerated testing. It maintains a high pH over time compared to normal cr...

materials and methods statistical and regressive modeling methods were used to investigate the effects of the carbon source and to optimize the production media. results the spinosad production of s. spinosa co121 increased 77.13%, from 310.44 ± 21.84 μg/ml in the initial fermentation medium (with glucose as the main carbon source) to 549.89 ± 38.59 μg/ml in a new optimized fermentation medium ...

BACKGROUND Saccharopolyspora spinosa is an important producer of antibiotic spinosad with clarified biosynthesis pathway but its complex regulation networks associated with primary metabolism and secondary metabolites production almost have never been concerned or studied before. The proteomic analysis of a novel Saccharopolyspora spinosa CCTCC M206084 was performed and aimed to provide a globa...

Spinosyns A and D are potent ingredient for insect control with exceptional safety to non-target organisms. It consists of a 21-carbon tetracyclic lactone with forosamine and tri-O-methylated rhamnose which are derived from S-adenosylmethionine. Although previous studies have revealed the involvement of metK1 (S-adenosylmethionine synthetase), rmbA (glucose-1-phosphate thymidylyltransferase), a...

The susceptibility of tsetse flies (Diptera: Glossinidae), Glossina palpalis gambiensis (Vanderplank), and G. m. morsitans (Westwood) to topically applied spinosad, a mixture of insecticidal molecules from the actinomycete Saccharopolyspora spinosa, is almost as high as to deltamethrin. However, susceptibility to spinosad does not differ significantly between teneral and gravid flies, contrary ...

BACKGROUND Spinosad (a mixture of spinosyns A and D) is a unique natural pesticide produced by Saccharopolyspora spinosa. With regard to attempts to improve S. spinosa by classical mutagenesis, we propose that the bottleneck of screening out high-spinosad-production strains is probably caused by the fermentation media. OBJECTIVES The current study aimed to identify a new medium to extensively...

This study verified the efficiency of ovitraps combined with Saccharopolyspora spinosa and Bacillus thuringiensis israelensis for monitoring Aedes aegypti albopictus in laboratory field conditions. In laboratory, A. aegypti, there was no difference eggs number between treatments grass infusions. For albopictus, average higher infusion. dry season, control. rainy infusion resulted a egg density,...

Background Creatine monohydrate (CrM) has been proven to be the most effective form of creatine and is considered the gold standard. However, a number of different forms of creatine have been purported to be more efficacious than CrM. The purpose of this study was to determine if a pH balanced form of creatine (Kre-Alkayn (KA), All American Pharmaceutical, Billings, MT, USA) that has been purpo...

Abstract Background Acetoin utilization protein (acuC) is a type I histone deacetylase which highly conserved in bacteria. The acuC gene related to the acetylation/deacetylation posttranslational modification (PTM) system S. spinosa. Spinosyns, secondary metabolites produced by Saccharopolyspora spinosa , are active ingredients family of insect control agents. However, specific functions and in...