This article reports the synthesis of a novel type of conjugate of three fundamental biological build blocks (i.e., saccharide, amino acids, and nucleobase) and their cell compatibility. The facile synthesis starts with the synthesis of nucleobase and saccharide derivatives, then uses solid-phase peptide synthesis (SPPS) to build the peptide segment (Phe-Arg-Gly-Asp or naphthAla-Phe-Arg-Gly-Asp...

A novel mechanism for cleavage of three typical α(1→2), α(1→4) and β(1→4) glucosidic bonds induced by hydroxyl free radical was examined with DFT theory at B3LYP/6-31+G(d,p) level using PCM water solvent model. It was found that the hydrogen abstraction from the CH bonds outside the saccharide rings could induce the hydrogen transfer from the hydroxyl at the radical carbon to the oxygen atom of...

The protective effects of saccharides with various molecular weights (glucose, maltose, maltotriose, maltotetraose, maltopentaose, maltoheptaose, dextran 1060, dextran 4900, and dextran 10200) against lyophilization-induced structural perturbation of model proteins (BSA, ovalbumin) were studied. Fourier transform infrared (FT-IR) analysis of the proteins in initial solutions and freeze-dried so...

Abstrac t . Changes in structure of a^-acid glycoprotein were followed after deglycosylation with neuraminidase, peptide N-glycohydrolase F or with a mixture of exoglycosidases Partially deglycosylated preparations of a i -ac id glycoprotein free of sialic acids, one complete saccharide component, sialic acids and one saccharide component and sialic acids and some of the external saccharides we...

The global consumption of herbs as medicine, nutraceuticals, food additives, cosmaceuticals, etc. is increasing rapidly. One of such area of high commercial potential is nonsaccharide sweeteners. Numerous compounds of plant origin are reported to have different degree of sweetness. In the light of limitations of currently marketed synthetic sweeteners as well as drastic reduction of high-calori...

Combination of bioaffinity and chromatography gave birth to affinity chromatography. A further combination with frontal analysis resulted in creation of frontal affinity chromatography (FAC). This new versatile research tool enabled detailed analysis of weak interactions that play essential roles in living systems, especially those between complex saccharides and saccharide-binding proteins. FA...

Polymeric synthetic host , azacrown-attached 2,6-pyridylene ethynylene polymer, was investigated for its saccharide recognition and the additive effect of triethylene tetramine-trifluoroacetic acid; heteroallosteric effects were observed on the basis of CD and UV/Vis analyses, which indicated saccharide-dependent stabilization and destabilization of helical complexes by the formation of pseudop...