A segment (hft) of bacteriophage FP43 DNA cloned into plasmid pIJ702 mediated high-frequency transduction of the resulting plasmid (pRHB101) by FP43 in Streptomyces griseofuscus. The transducing particles contained linear concatemers of plasmid DNA. Lysates of FP43 prepared on S. griseofuscus containing pRHB101 also transduced many other Streptomyces species, including several that restrict pla...

antibiotics are the best known products of actinomycete. over 5,000 antibiotics have been identified from the cultures of gram positive and gram-negative organisms, and filamentous fungi, but only about 100 antibiotics have commercially been used to treat human, animal and plant diseases. the genus, streptomycete, is responsible for the formation of more than 60% of known antibiotics. bacterial...

A strain of Streptomyces griseofuscus S42227 (FERM P-8443) was found to produce new antitumor antibiotics, called azinomycins A and B. The molecular formulas of azinomycins A and B were determined as C30H33N3O10 and C31H33N3O11, respectively. They were active against Gram-positive bacteria, Gram-negative bacteria and L5178Y cells in tissue culture.

Die enantioselektive Synthese bioaktiver Verbindungen, die ein Pyrroloindolgerüst tragen, ist oft aufwändig. Im Gegensatz dazu sind mehrere S-Adenosylmethionin (SAM)-abhängige Methyltransferasen bekannt, stereo- und regioselektive Methylierungen an der C3-Position verschiedener Indole durchführen damit eine direkte Bildung des gewünschten Pyrroloindolgerüsts ermöglichen. Hier wird SAM-abhängige...