the hepatitis b surface antigen ( hbsag) gene was isolated from an iranian hbeag positive carrier by pcr. the gene was cloned in puc19 for sequencing and pyes2 for expression in saccharomyces cerevisiae, which pnf1 and pdf3 constructs were made respectively. the sequencing data showed that the isolated hbsag gene shared more than 90% homology with the ayw subtype. the pdf3 was transferred into ...

Vitamin D deficiency causes osteoporosis, osteopenia, fractures, rickets and more recently is linked with some chronic illnesses such as cancer. Because of the safety and probiotic properties of the yeast Saccharomyces cerevisiae, we hypothesized that yeast cells enriched with cholecalciferol (vitamin D3) could represent a solution for prevention or treatment of vitamin D deficiency. In this st...

Background and Aim: Pseudomonas aeruginosa is an important ubiquitous and especially common pathogen in the hospital. Exotoxin A that encoded by exoA gene has a role in pathogenesis of this bacterium. Today, probiotics are widely used in the treatment and prevention of diseases. The present study aimed to study the Saccharomyces cerevisiae S3 effect on the expression of exoA gene. Materials an...

Vitamin D deficiency causes osteoporosis, osteopenia, fractures, rickets and more recently is linked with some chronic illnesses such as cancer. Because of the safety and probiotic properties of the yeast Saccharomyces cerevisiae, we hypothesized that yeast cells enriched with cholecalciferol (vitamin D3) could represent a solution for prevention or treatment of vitamin D deficiency. In this st...

Strain Genotype Source BY4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Open Biosystems W303 MATa ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 doa4 (BY4741) MATa doa4::KanMX4 Open Biosystems MHY753 MATa his3-Δ200 leu2D1 ura3-52 lys2-801 trp1-63 ade2-101 [19] MHY754 MATa his3-Δ200 leu2D1 ura3-52 lys2-801 trp1-63 ade2-101 cim3-1 [19] YKA247 (W303) bar1::URA3 acm1::KanMX4 [11] YKA404 MATα acm1::Na...

The Biosorption property of S. cerevisiae for chromium uptake was investigated in an immobilized cell bioreactor. Saw dust was utilized as the solid bed in the reactor. Adsorption of S. cerevisiae on saw dust obeys a first order reaction kinetic up to 6 hours. The immobilized biomass particles are porous and exist in the new generation of biological adsorbent. Chromium biosorption was studied i...

BACKGROUNDS: Optimization of microbial compositions and load in live food during the process of bioencapsulation is one of the most important concerns in aquaculture, as it can promote the growth and feeding parameters of fish larvae. OBJECTIVES: The aim of this study was to determine the growth and feeding performance of Persian sturgeon larvae fed with bioencapsulated D. magna with Saccharomy...