The hydrolysis of ochratoxin A (OA) and the corresponding formation of its hydrolysis product, alpha ochratoxin (O alpha), by ruminal digesta and in the rumen of hay-fed and grain-fed sheep were compared. Ruminal contents from sheep fed diets with hay or with grain hydrolyzed OA in vitro; the majority of the activity was associated with the particulate fraction of the ruminal contents. The rate...

This experiment was carried out to evaluate the effect of specific gravity (SG) and particle size (PS) of Chromium-mordanated alfalfa neutral detergent fiber (NDF) markers on estimation of particulate rumen turnover. Thirty-two multiparous, mid lactation Holstein dairy cows (body weight=654±24 kg) were allotted to a completely randomized design experiment with two replications in a 4×4 factoria...

The objective of this experiment was to investigate the possibility of estimating the outflow of nutrients and microbial protein from the rumen based on sampling reticular contents as an alternative to duodenal sampling. Microbial protein flow estimates were also compared with a third method based on sampling of ruminal contents. Reticular and duodenal digesta and ruminal contents were recovere...

Two experiments were conducted to study the effects of methionine supplementation on ruminal fermentation and digesta kinetics. In Exp. 1, nine ruminally cannulated beef heifers (average initial BW = 527 kg) in a crossover design were fed low-quality grass hay and cottonseed meal with or without 11.4 g of supplemental methionine (polysaccharide-coated). Particulate and fluid kinetics, rate of D...

. eight holstein cows in early lactating stage were used in a 4×4 change-over design within 4 periods of 21-d to determine the effects of alfalfa hay particle size and soybean oil supplementation on intake, digestibility, ruminal and blood metabolites, chewing activity, milk yield and compositions. four total mixed rations containing 20, 20 and 60% corn silage, alfalfa hay and concentrate respe...

Two experiments were conducted to determine effects of sodium bicarbonate (NaHCO3) on nitrogen (N) balance, ruminal N metabolism and site and extent of nutrient digestion in sheep fed 75% concentrate diets. A 2 X 2 factorial arrangement of treatments was employed in both trials with experimental diets balanced for 10.5 or 12.0% crude protein and containing 0 or 3.5% NaHCO3. In experiment 1, 12 ...

Describing routes is an easy everyday task for people who know the environment well. However, strategies exist also for situations where only incomplete knowledge is available. We present a study eliciting verbalized route plans and thinkaloud data from novices and experts who were asked to find and describe routes in a complex building. The spatial descriptions were analyzed relating the level...

The stability and the partitioning of closantel and rafoxanide in ruminal fluid (RF) was examined in vitro. Stability was evaluated in two studies in a ruminal fluid-artificial saliva (RF-AS) mixture containing either drug. Drug concentrations were measured in samples collected sequentially from four batches of RF-AS fortified with either closantel or rafoxanide in one study and in four separat...

Five crossbred wethers (58 kg) fitted with cannulas in the rumen, duodenum, and ileum were used in a 5 x 5 Latin square design to study effects of feeding combinations of alkaline hydrogen peroxide-treated wheat straw (AHP-WS) and alfalfa hay at restricted intakes on site and extent of nutrient digestion. Additionally, flows and disappearance of N and amino acids (AA) in the small intestine wer...