rumen is a persistent and specific ecosystem consists of bacteria, protozoa and fungus where feed fermentation takes place in it. produced hydrogen in rumen can be used in the synthesis of the volatile fatty acids and the microbial protein and its excess would be eliminated through the production of methane by methanogenesis. nutritionists have tried to find ways to decrease loss and energy and...

Cheviot wethers were defaunated by using dioctyl sodium sulfosuccinate and were constantly infused with urea to provide 2.87% of the daily N intake. Defaunation resulted in higher rumen dry matter and lower rumen pH. The molar per cent propionate was higher in defaunated sheep, whereas the molar per cent butyrate and acetate was lower. Apparent nitrogen digestibility, nitrogen utilization, and ...

1. The rate of transfer of plasma urea-nitrogen to rumen ammonia was measured by infusion of 15NH4Cl and [15N]urea into sheep given brome grass (Bromus inermis) or lucerne (Medicago sativa) pellets. Urea was infused into the rumen or abomasum of two sheep given brome grass in order to increase the concentration of rumen ammonia. 2. From 6.2 to 9.8 g/d of plasma urea-N were transferred to the ru...

This study was conducted to investigate the effects of different levels of peptide supplementation on rumen fermentation pattern, digestibility and microbial protein synthesis. Three rumen-cannulated Holstein dairy cows were used in a 3 × 3 Latin square experiment within 21 days period. The ruminal infusion of sodium caseinate (CN) was 0 (control), 50 and 100 g/d. Dry matter intake, milk yield ...

Dry matter intake, nitrogen intake, nitrogen digestibility, rumen ammonia-N and plasma urea-N concentration were measured in young rusa deer fed 4 diets containing 90 to 205 g/kg DM crude protein. The level of crude protein associated with maximal voluntary food intake and dry matter digestibility for these animals was found to be about 160 g/kg DM. Plasma urea-N was positively related to rumen...

This study was conducted to determine in vitro crude protein degradation (IVDP) parameters and effective crude protein degradability (EPD) of various feeds using the modified in vitro gas production (GP) technique. Feed samples were alfalfa hay, soybean meal, soybean, rapeseed meal, sunflower meal and fish meal. Rumen fluid was collected before the morning feeding from four rumen fistulated lam...

Urea, a non-protein nitrogen for dairy cows, is rapidly hydrolyzed to ammonia by urease produced by ureolytic bacteria in the rumen, and the ammonia is used as nitrogen for rumen bacterial growth. However, there is limited knowledge with regard to the ureolytic bacteria community in the rumen. To explore the ruminal ureolytic bacterial community, urea, or acetohydroxamic acid (AHA, an inhibitor...

An experiment was carried out to investigate the dynamics of nitrogen (N) in the rumen and abomasum of rumen and abomasum-cannulated sheep using 15N dilution techniques. The 15N tracer was administered into the rumen as 15N-ammonia or 15N-labelled duckweed and the transfer of the 15N label to various N pools was followed. Flow of digesta from the rumen into the abomasum was ascertained by doubl...

this study was conducted to determine in vitro crude protein degradation (ivdp) parameters and effective crude protein degradability (epd) of various feeds using the modified in vitro gas production (gp) technique. feed samples were alfalfa hay, soybean meal, soybean, rapeseed meal, sunflower meal and fish meal. rumen fluid was collected before the morning feeding from four rumen fistulated lam...

Relationships between ammonia levels in rumen liquor and dietary nitrogen (N) intake, the N concentration in the diet, blood urea concentration and urinary N excretion were examined in sheep fed near ad libitum on diets varying widely in chemical composition and digestibility. Rumen ammonia was positively correlated with all measures but the best relationship was with the ratio of N to digestib...