abdelbasset amara unit of molecular endocrinology, sousse faculty of medicine, university of sousse, sousse, tunisia ; laboratory of human cytogenetics, molecular genetics and reproductive biology, farhat hached university hospital, sousse, tunisia ; higher institute of biotechnology of monastir, university of monastir, tunisia. ilhem ben charfeddine laboratory of human cytogenetics, molecular genetics and reproductive biology, farhat hached university hospital, sousse, tunisia ; higher institute of biotechnology of monastir, university of monastir, tunisia. houda ghédir laboratory of human cytogenetics, molecular genetics and reproductive biology, farhat hached university hospital, sousse, tunisia ; higher institute of biotechnology of monastir, university of monastir, tunisia. ons mamaï laboratory of human cytogenetics, molecular genetics and reproductive biology, farhat hached university hospital, sousse, tunisia ; higher institute of biotechnology of monastir, university of monastir, tunisia. saloua jemni-yacoub regional center of blood transfusion of sousse, sousse, tunisia. larbi chaieb dept. of endocrinology and diabetology, farhat hached university hospital, sousse, tunisia.