embryonic stem cells are capable of differentiating to variety of cell tissues including cardiomyocytes. this developmental change is accompanied with a great deal of ion channel expression and functions. mouse stem cell derived cardiomyocytes were prepared and separated to yield isolated single cell suspension for cell current recording. in the present study some properties of the k + -current...

introduction: embryonic stem (es) cells are pluripotent cells derived from inner cell mass of blastocyst. these cells can product any cell by spontaneous or directed differentiation. this study was started to produce neuron cells by directed differentiation of new murine es cell line in vitro. materials and methods: murine es cells (royan b1 derived from c57bl/6 strain) were used for in vitro n...

BACKGROUND In recent years the increasing use of nanoparticles has led researchers to study their effects on biological systems. The most important effects of nanoparticles on cells are their ability to induce or suppress production of reactive oxygen species (ROS). Changes in reactive oxygen species play an important role in various developmental processes, including proliferation and differen...

1. Department of Biological Sciences, Kharazmi University, Tehran, Iran. 2. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. 3. MSc student in Developmental Biology Sciences, Kharazmi University, Tehran, Iran. 4. BSc in Biology, Reproductive Biomedicine Research Center, Royan Institute fo...

introduction: the aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. methods: in order to produce embryoid bodies, es cells line royan b1 were grown in suspension in the absence of lif for 4 days. ebs were divided into 4 groups. ebs in group 1 & 2 were further cultured i...

Introduction: The aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. Methods: In order to produce embryoid bodies, ES cells line Royan B1 were grown in suspension in the absence of LIF for 4 days. EBs were divided into 4 groups. EBs in group 1 & 2 were further cultur...

introduction: embryonic stem (es) cells are pluripotent cells derived from the inner cell mass of blastocysts. these cells are appropriate for creation of animal models of human genetic diseases, the study of gene functions in vivo and differentiation into specific types as potential therapeutic agents for several diseases. we describe here production of transgenic chimeric mouse blastocysts by...

Introduction Cholinergic system is one of the important systems of mammalian CNS. Cholinergic neurons distributed in brain and spinal cord and contributed to principal functions like: consciousness, learning and memory, and motor control. In this study we investigated the differentiation potentiality of mouse embryonic stem cells toward cholinergic neurons. The aim of this study was to evaluate...