نتایج جستجو برای: rolc

تعداد نتایج: 73  

Journal: :The European respiratory journal 2010
A B Taegtmeyer C Zettler M Siegemund D A Tsakiris A E Rätz Bravo H Pargger S Kraehenbuehl M Haschke

In conclusion, although surgery remains the first choice to treat ROLC, a considerable number of patients are inoperable. EBRT is an alternative, but is hampered since the ROLC is effectively invisible. We report that the use of metallic clips placed during bronchoscopy can make ROLC visible for the radiation oncologist. The clips appear to be safe, well tolerated and removable in inoperable pa...

2002
Nobuharu Fujii Ryusuke Yokoyama Hirofumi Uchimiya

In cell cultures of carrot (Daucus carota l.), somatic embryogenesis can be induced by transferring cells from a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) to one devoid of 2,4-D. Previous analysis of transgenic carrot cells containing the 5' noncoding sequence of the Ri plasmid rolC and a structural gene for bacterial 0-glucuronidase (uidA) has shown that the chimeric gene is act...

Journal: :Plant physiology 1994
N Fujii R Yokoyama H Uchimiya

In cell cultures of carrot (Daucus carota L.), somatic embryogenesis can be induced by transferring cells from a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) to one devoid of 2,4-D. Previous analysis of transgenic carrot cells containing the 5' non-coding sequence of the Ri plasmid rolC and a structural gene for bacterial beta-glucuronidase (uidA) has shown that the chimeric gene is...

Journal: :The Plant cell 1989
A Spena R B Aalen S C Schulze

We describe a genetic switch based on the Ac transposable element of maize and the rolC gene of Agrobacterium rhizogenes, a dominant gene, which has pleiotropic effects on plant growth and morphology. Moreover, rolC gene expression under the control of the 35S cauliflower mosaic virus promoter decreases chlorophyll content in transgenic tobacco plants. Chlorophyll is a visible cell-autonomous m...

2014
Luybov A. Wasserman Nina P. Aksenova Tatiyana N. Konstantinova Lidiya I. Sergeeva Svetlana A. Golyanovskaya Alexey V. Krivandin Georgy A. Romanov

Wild-type potato (Solanum tuberosum L.) plants and their transformants harboring agrobacterial rolB or rolC genes under control of the patatin class I promoter were cultured in vitro. These plants were used as a source of single-node stem cuttings. The structure of native starch in tubers formed on cuttings was determined using methods of X-ray scattering and differential scanning microcalorime...

Journal: :Chest 2001
T G Sutedja H Codrington E K Risse R H Breuer J C van Mourik R P Golding P E Postmus

BACKGROUND The ability of conventional CT scans and fiberoptic bronchoscopy to localize and properly stage radiographically occult lung cancer (ROLC) in the major airways is limited. High-resolution CT (HRCT) scanning and autofluorescence bronchoscopy (AFB) may improve the assessment of ROLC before the most appropriate therapy can be considered. PATIENTS AND METHODS We prospectively studied 2...

ژورنال: :مهندسی ژنتیک و ایمنی زیستی 0
امین سهندی خلیفه کندی amin sahandi [email protected] مقصود پژوهنده maghsoud pazhouhandeh biotechnology dept. azarbaijan shahid madani universityگروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان هانیه محجل شجا hanieh mohajjel shoja گروه زیست شناسی، دانشکده علوم طبیعی، دانشگاه تبریز

پروانش (catharanthus roseus) از مهم­ترین گیاهان دارویی بوده و دارای آلکالوئید­های بسیار مهم از جمله وین­بلاستین و وین­کریستین می­باشد که در درمان انواع سرطان­ها کاربرد دارد. با توجه به اهمیت و نقش ژنهای پلاستیسیتی مثل rolc در بیوسنتز آلکالوئیدها، در این پژوهش به بررسی بهبود ریشه دهی و القای ریشه در گیاه دارویی پروانش  به کمک این ژن پرداخته شد. برای این منظور بذرهای این گیاه، استریل شده و در شرا...

پروانش (Catharanthus roseus) از مهم­ترین گیاهان دارویی بوده و دارای آلکالوئید­های بسیار مهم از جمله وین­بلاستین و وین­کریستین می­باشد که در درمان انواع سرطان­ها کاربرد دارد. با توجه به اهمیت و نقش ژنهای پلاستیسیتی مثل rolC در بیوسنتز آلکالوئیدها، در این پژوهش به بررسی بهبود ریشه دهی و القای ریشه در گیاه دارویی پروانش  به کمک این ژن پرداخته شد. برای این منظور بذرهای این گیاه، استریل شده و در شرا...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1991
G Hansen M Larribe D Vaubert J Tempé B J Biermann A L Montoya M D Chilton J Brevet

This paper presents the map and DNA sequence analysis of pRi8196 transferred DNA (T-DNA) genes encoding root-inducing and mannopine synthesis functions. A canonical 24-base-pair border repeat as well as two "pseudoborders" are present at the functional right T-DNA border. To the left of this border are homologs of the mas1' and mas2' genes of TR pRiA4. Next to these are five open reading frames...

Journal: :Zeitschrift fur Naturforschung. C, Journal of biosciences 2010
Hedviga Komarovská Ján Kosuth Annalisa Giovannini Andrija Smelcerovic Sebastian Zuehlke Eva Cellárová

The extent of phenotypic variation of St. John's wort (Hypericum perforatum L.) plants transformed with wild agropine ATCC 15834 Agrobacterium rhizogenes plasmid was evaluated with respect to the number of rol genes integrations. The transfer of T(L)-DNA to plant explants during each transformation event was incomplete with different rolA, rolB, and rolC copy numbers. Along with typical feature...

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