Toxoplasmosis is an important zoonotic disease that can cause abortion in humans and animals. The aim of this study was isolation and subsequent genotyping of Toxoplasma gondii isolates in ovine aborted fetuses. During 2012-2013, 39 ovine aborted fetuses were collected from sheep flocks in Khorasan Razavi Province, Iran. The brain samples were screened for detection of the parasite DNA by neste...

Celem badań było określenie jakości tusz wieprzowych oraz cech fizycznych i składu chemicznego polędwicy szynki w odniesieniu do polimorfizmu locus GH. W doświadczeniu wykorzystano tuczniki mieszańce ras: wielkiej białej polskiej, polskiej zwisłouchej, duroc pietrain, utrzymywane gospodarstwie indywidualnym na terenie woj. podkarpackiego. Polimorfizm genu GH oznaczono metodą PCR-RLFP, wykorzyst...

Deep hypothermic circulatory arrest (DHCA) is commonly used for neonatal cardiac surgery. However, prolonged exposure to DHCA is associated with neurologic morbidity. The Norwood operation and aortic arch advancement are procedures that typically require DHCA during surgical correction. Regional low flow perfusion (RLFP) can be used to limit or exclude the use of circulatory arrest. This techni...

Objective: The aim of the study was to analyze a series of Brazilian patients suffering from MELAS. Method: Ten patients with MELAS were studied with correlation between clinical findings, laboratorial data, electrophysiology, histochemical and molecular features. Results: Blood lactate was increased in eight patients. Brain image studies revealed a stroke-like pattern in all patients. Muscle b...

IGFBP-3 is responsible for the multiple effects of growth factors in most mammalian species and considered major transport factor growth, used as a marker different body functions such metabolism, reproduction, weight control, immunity, energy balance, so on. Considered candidate gene, production traits its essential role development animals. For DNA extraction, blood samples are obtained jugul...

An assessment was made of the genetic variation of the Pulawska pig through the determination of polymorphism of 6 genes and 14 microsatellite sequences. The examinations covered 52 gilts included in a preservation breeding project. The identification of the alleles at microsatellite loci was performed in an ABI PRISM 310 GENETIC ANALYZER. Gene polymorphism was established by the PCR-RLFP metho...

Methods A cross-sectional study of 223 randomly selected HIVinfected outpatients was undertaken. Recruitment was stratified by gender for age groups 30-39, 40-49 yrs and ≥50 yrs. Osteopenia and osteoporosis were diagnosed according to the WHO criteria. Patients completed a detailed questionnaire including combination HIV drugs (cART) history, & a dual-energy X-ray absorptiometry (DEXA) of Lumba...

Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of...