نتایج جستجو برای: reverse transcription

تعداد نتایج: 334537  

Journal: :iranian journal of virology 0
f heidari department of plant protection, faculty of agricultural sciences and engineering, college of agriculture and natural resources, university of tehran, karaj, iran m koohi-habibi department of plant protection, faculty of agricultural sciences and engineering, college of agriculture and natural resources, university of tehran, karaj, iran gh mosahebi department of plant protection, faculty of agricultural sciences and engineering, college of agriculture and natural resources, university of tehran, karaj, iran

background and aims: the causal agents of viral lettuce big vein disease are two viruse, lettuce big vein associated virus (varicosavirus) and mirafiori lettuce virus (ophiovirus). these viruses have coat proteins of similar size but have different morphologies and serologically unrelated.the purpose of this study was to distinguish and detect lbvav and milv in lettuce fields in tehran province...

Journal: :iranian journal of virology 0
p mehrbod influenza unit pasteur institute of iran, tehran, iran e amini influenza unit pasteur institute of iran, tehran, iran m tavassoti-kheiri influenza unit pasteur institute of iran, tehran, iran

background and aims: influenza virus is the most important cause of annual morbidities and mortalities worldwide with numerous antigenic drifts and shifts. inaccessibility to effective drugs and vaccines has made world health authorities to be interested in traditional medicine in order to prevent spread of the infectious agent. garlic is one of the most famous of all plants in human history. i...

Journal: :archives of pediatric infectious diseases 0
roya nikfar department of pediatric infectious diseases, aboozar children’s hospital, ahvaz jundishapur university of medical sciences, ahvaz, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences) ahmad shamsizadeh infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-9161136128, fax: +98-6114433715 ; infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-9161136128, fax: +98-6114433715سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences) manoochehr makvandi department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences) arash khoshghalb department of pediatric infectious diseases, aboozar children’s hospital, ahvaz jundishapur university of medical sciences, ahvaz, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences)

conclusions: this study indicates that rsv is a substantial cause of respiratory tract infection in infants less than 2 years old. rt-pcr offers a rapid method for detection of rsv in hospitalized children with lrti. results: during the study period, 9 children had a positive result for rsv infection. the median age of these patients was 10 months. bronchiolitis was the clinical diagnosis of pa...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه الزهراء - دانشکده علوم پایه 1391

فرایند وارونویسی (reverse transcription)همراه با ازدیاد (rt-pcr) dna روش قوی مولکولی است که برای تشخیص، تعیین کمیت و بررسی بیان ژن و ویروس های rna دار، کاربرد بسیاری دارد. کارایی این واکنش ها بیشتر به ویژگی های مولکولی آنزیم های وارونوشتاز(reverse transcriptase) موجود وابسته است. ساخت cdna در دمای بالا مزیت های بسیاری دارد از جمله حذف ساختارهای دوم rna و بنابر این وارونوشتازهای پایـدار حرارتی، ...

Journal: :iranian biomedical journal 0
محمدرضا رضوانی mohammad reza rezvany اعظم کاظمی azam kazemi عباس حاجی فتحعلی abbas hajifathali سعید کاویانی saeed kaviani håkan hakan mellstedt

the human leukocyte antigen g (hla-g) molecule exhibits limited tissue distribution, low polymorphism and alternative splicings that generate seven hla-g isoforms. hla-g exerts multiple immunoregulatory functions. recent studies indicate an ectopic up-regulation in tumor cells that may favor their escape from anti-tumor immune responses. this study it is an effort to clarify the presence of hla...

ژورنال: :مجله دانشکده پزشکی اصفهان 0
شهناز رضوی استاد، گروه علوم تشریحی و بیولوژی مولکولی، دانشکده ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران نفیسه احمدی کارشناس ارشد، گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، تهران، ایران محمد کاظمی دانشجوی دکتری، گروه ژنتیک، دانشکده ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران حمیدرضا صادقیان دانشجوی پزشکی، دانشکده ی پزشکی و کمیته ی تحقیقات دانشجویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

مقدمه: با توجه به سهولت دستیابی به سلول های بنیادی حاصل از بافت چربی نسبت به سایر منابع سلول های بنیادی در صورتی که بتوان از عوامل رشد مانند استروژن جهت افزایش تمایز نرونی استفاده نمود، نرون های تمایز یافته، در پیوند اتولوگ بیماری های عصبی دژنراتیو خاص مانند بیماری پارکینسون و ضایعات نخاعی استفاده ی وسیعی دارد. هدف از این مطالعه، ارزیابی تأثیر استروژن بر تمایز عصبی سلول های بنیادی مشتق از بافت ...

Journal: :iranian journal of virology 0
f sheikholeslami virology department, pasteur institute of iran, tehran, iran h khanahmadi genetic department, faculty of medical sciences of isfahan university, isfahan, iran m ajorloo virology departments, faculty of medicine, tarbiat modares university, tehran, iran r ahangari cohan pilot nano biotechnology department, pasteur institute of iran, tehran, iran z karimi department of pharmaceutical biotechnology and pharmaceutical sciences research center, school of pharmacy, shiraz university of medical sciences, shiraz, iran d norouzian pilot nano biotechnology department, pasteur institute of iran, tehran, iran

vaccines is considered as a critical point and in-process of quality control (ipqc) test of vaccine production. rapid tests, like real time pcr, are more appropriated when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. in the current study, a real-time quantitative reverse transcription-polymerase chain reaction (...

Journal: :international journal of infection 0
nawfal rasheed hussein department of internal medicine, school of medical sciences, faculty of medicine, university of duhok, duhok, iraq; department of internal medicne, school of medicine, faculty of medical sciences, university of duhok, duhok, iraq sefar mohamad haj department of internal medicine, school of medical sciences, faculty of medicine, university of duhok, duhok, iraq lokman amin almizori department of internal medicine, school of medical sciences, faculty of medicine, university of duhok, duhok, iraq ali ahmed taha the department of infection control, general directorate of health-duhok, duhok, iraq

conclusions the prevalence of hbv infection among donors in duhok was comparable with those of reports from other parts of iraq. education and immunization should be initiated to target the high-risk groups. furthermore, a community-based study is recommended to study the prevalence of hbv and hcv infection in the population. background the prevalence of blood borne viral infection is escalatin...

Journal: :avicenna journal of medical biotechnology 0

background: acute promyelocytic leukemia (apl) is a subclass of acute myeloid leukemia. the chromosomal aberration in 95% of apl cases is t(15; 17) (q22; q21), which prevents cell differentiation. characterization of the underlying molecular lesion is valuable in determining optimal treatment strategy. the goal of this study was to develop a new and powerful flow- fish technique to detect the l...

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