Retrotransposon-based molecular markers are powerful molecular tools. However, these markers are not readily available due to the difficulty in obtaining species-specific retrotransposon primers. Although recent techniques enabling the rapid isolation of retrotransposon sequences have facilitated primer development, this process nonetheless remains time-consuming and costly. Therefore, research...

Retrotransposons are highly prevalent in mammalian genomes due to their ability to amplify in pluripotent cells or developing germ cells. Host mechanisms that silence retrotransposons in germ cells and pluripotent cells are important for limiting the accumulation of the repetitive elements in the genome during evolution. However, although silencing of selected individual retrotransposons can be...

Retrotransposon sequences are positioned throughout the genome of almost every eukaryote that has been sequenced. As mobilization of these elements can have detrimental effects on the transcriptional regulation and stability of an organism's genome, most organisms have evolved mechanisms to repress their movement. Here, we identify a novel role for the Drosophila melanogaster Condensin II subun...

Abstract. Fatmawati Y, Setiawan AB, Purwantoro A, Respatie DW, Teo CH. 2021. Analysis of genetic variability in F2 interspecific hybrids mung bean (Vigna radiata) using inter-retrotransposon amplified polymorphism marker system. Biodiversitas 22: 4880-4889. Mung radiata L. Wilczek) categorized as one the pivotal annual crops Vigna genera is commonly cultivated rotation with cereal during drough...

Investigating Genetic Diversity and Relationships in some Henbane (Hyoscyamus spp.) Populations Based on Polymorphisms Resulting from Retrotransposon Insertion

Mammalian genomes harbor millions of retrotransposon copies, some of which are transpositionally active. In mouse prospermatogonia, PIWI-interacting small RNAs (piRNAs) combat retrotransposon activity to maintain the genomic integrity. The piRNA system destroys retrotransposon-derived RNAs and guides de novo DNA methylation at some retrotransposon promoters. However, it remains unclear whether ...

There is an increased global outbreak of diseases caused by coronaviruses affecting respiratory tracts birds and mammals. Recent dangerous are MERS-CoV, SARS-CoV, SARS-CoV-2, causing illness even failure several organs. However, profound impact coronavirus on host cells remains elusive. In this study, we analyzed transcriptome SARS-CoV-2 infected human lung-derived cells, observed that infectio...

tempranillo is one of the most widely cultivated grapevine varieties in spain. after several years of clone selection, some highly recommended old clones have been identified in terms of both their qualitative and production characteristics. this study was designed to discriminate among 28 ancient clones of the cultivar tempranillo (vitis vinifera). dna samples from clones were analysed using t...

The wx-K mutation results from the insertion of a copia-like retrotransposon into exon 12 of the maize waxy gene. This retrotransposon, named Hopscotch, has one long open reading frame encoding all of the domains required for transposition. Computer-assisted database searches using Hopscotch and other plant copia-like retroelements as query sequences have revealed that ancient, degenerate retro...

In the human placenta, DNA hypomethylation permits the expression of retrotransposon-derived genes that are normally silenced by methylation in somatic tissues. We previously identified hypomethylation of a retrotransposon-derived transcript of the voltage-gated potassium channel gene KCNH5 that is expressed only in human placenta. However, an RNA sequence from this placental-specific transcrip...